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Science 325 (5940): 585-590

Copyright © 2009 by the American Association for the Advancement of Science

Pre-Target Axon Sorting Establishes the Neural Map Topography

Takeshi Imai,1,* Takahiro Yamazaki,1,* Reiko Kobayakawa,1 Ko Kobayakawa,1 Takaya Abe,2 Misao Suzuki,3 Hitoshi Sakano1,{dagger}

Abstract: Sensory information detected by the peripheral nervous system is represented as a topographic map in the brain. It has long been thought that the topography of the map is determined by graded positional cues that are expressed by the target. Here, we analyzed the pre-target axon sorting for olfactory map formation in mice. In olfactory sensory neurons, an axon guidance receptor, Neuropilin-1, and its repulsive ligand, Semaphorin-3A, are expressed in a complementary manner. We found that expression levels of Neuropilin-1 determined both pre-target sorting and projection sites of axons. Olfactory sensory neuron–specific knockout of Semaphorin-3A perturbed axon sorting and altered the olfactory map topography. Thus, pre-target axon sorting plays an important role in establishing the topographic order based on the relative levels of guidance molecules expressed by axons.

1 Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan.
2 Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan.
3 Division of Transgenic Technology, Center for Animal Resources and Development, Kumamoto University, Kumamoto 860-0081, Japan.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: sakano{at}mail.ecc.u-tokyo.ac.jp

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