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Science 325 (5945): 1250-1254

Copyright © 2009 by the American Association for the Advancement of Science

Rab35 Controls Actin Bundling by Recruiting Fascin as an Effector Protein

Jun Zhang,1 Marko Fonovic,2,3 Kaye Suyama,1 Matthew Bogyo,2 Matthew P. Scott1,*

Abstract: Actin filaments are key components of the eukaryotic cytoskeleton that provide mechanical structure and generate forces during cell shape changes, growth, and migration. Actin filaments are dynamically assembled into higher-order structures at specified locations to regulate diverse functions. The Rab family of small guanosine triphosphatases is evolutionarily conserved and mediates intracellular vesicle trafficking. We found that Rab35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells. These effects were mediated by the actin-bundling protein fascin, which directly associated with active Rab35. Targeting Rab35 to the outer mitochondrial membrane triggered actin recruitment, demonstrating a role for an intracellular trafficking protein in localized actin assembly.

1 Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
2 Department of Pathology and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
3 Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan Institute, Jamova ulica 39, 1000 Ljubljana, Slovenia.

* To whom correspondence should be addressed. E-mail: mscott{at}stanford.edu

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