Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 325 (5945): 1250-1254

Copyright © 2009 by the American Association for the Advancement of Science

Rab35 Controls Actin Bundling by Recruiting Fascin as an Effector Protein

Jun Zhang,1 Marko Fonovic,2,3 Kaye Suyama,1 Matthew Bogyo,2 Matthew P. Scott1,*

Abstract: Actin filaments are key components of the eukaryotic cytoskeleton that provide mechanical structure and generate forces during cell shape changes, growth, and migration. Actin filaments are dynamically assembled into higher-order structures at specified locations to regulate diverse functions. The Rab family of small guanosine triphosphatases is evolutionarily conserved and mediates intracellular vesicle trafficking. We found that Rab35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells. These effects were mediated by the actin-bundling protein fascin, which directly associated with active Rab35. Targeting Rab35 to the outer mitochondrial membrane triggered actin recruitment, demonstrating a role for an intracellular trafficking protein in localized actin assembly.

1 Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
2 Department of Pathology and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
3 Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan Institute, Jamova ulica 39, 1000 Ljubljana, Slovenia.

* To whom correspondence should be addressed. E-mail: mscott{at}stanford.edu


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mediation of Clathrin-Dependent Trafficking during Cytokinesis and Cell Expansion by Arabidopsis STOMATAL CYTOKINESIS DEFECTIVE Proteins.
C. M. McMichael, G. D. Reynolds, L. M. Koch, C. Wang, N. Jiang, J. Nadeau, F. D. Sack, M. B. Gelderman, J. Pan, and S. Y. Bednarek (2013)
PLANT CELL 25, 3910-3925
   Abstract »    Full Text »    PDF »
Rab35 regulates cadherin-mediated adherens junction formation and myoblast fusion.
S. Charrasse, F. Comunale, S. De Rossi, A. Echard, and C. Gauthier-Rouviere (2013)
Mol. Biol. Cell 24, 234-245
   Abstract »    Full Text »    PDF »
Molecular Mechanism of Fascin Function in Filopodial Formation.
S. Yang, F.-K. Huang, J. Huang, S. Chen, J. Jakoncic, A. Leo-Macias, R. Diaz-Avalos, L. Chen, J. J. Zhang, and X.-Y. Huang (2013)
J. Biol. Chem. 288, 274-284
   Abstract »    Full Text »    PDF »
Fascin controls neuronal class-specific dendrite arbor morphology.
J. Nagel, C. Delandre, Y. Zhang, F. Forstner, A. W. Moore, and G. Tavosanis (2012)
Development 139, 2999-3009
   Abstract »    Full Text »    PDF »
Rab35 regulates Arf6 activity through centaurin-{beta}2 (ACAP2) during neurite outgrowth.
H. Kobayashi and M. Fukuda (2012)
J. Cell Sci. 125, 2235-2243
   Abstract »    Full Text »    PDF »
Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton.
A. L. Marat, M. S. Ioannou, and P. S. McPherson (2012)
Mol. Biol. Cell 23, 163-175
   Abstract »    Full Text »    PDF »
Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during Fc{gamma}R-mediated phagocytosis.
Y. Egami, M. Fukuda, and N. Araki (2011)
J. Cell Sci. 124, 3557-3567
   Abstract »    Full Text »    PDF »
EPI64 Protein Functions as a Physiological GTPase-activating Protein for Rab27 Protein and Regulates Amylase Release in Rat Parotid Acinar Cells.
A. Imai, S. Yoshie, K. Ishibashi, M. Haga-Tsujimura, T. Nashida, H. Shimomura, and M. Fukuda (2011)
J. Biol. Chem. 286, 33854-33862
   Abstract »    Full Text »    PDF »
Mechanism of Actin Filament Bundling by Fascin.
S. Jansen, A. Collins, C. Yang, G. Rebowski, T. Svitkina, and R. Dominguez (2011)
J. Biol. Chem. 286, 30087-30096
   Abstract »    Full Text »    PDF »
DENN Domain Proteins: Regulators of Rab GTPases.
A. L. Marat, H. Dokainish, and P. S. McPherson (2011)
J. Biol. Chem. 286, 13791-13800
   Abstract »    Full Text »    PDF »
Role of Rab GTPases in Membrane Traffic and Cell Physiology.
A. H. Hutagalung and P. J. Novick (2011)
Physiol Rev 91, 119-149
   Abstract »    Full Text »    PDF »
Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C.
C. Hsu, Y. Morohashi, S.-i. Yoshimura, N. Manrique-Hoyos, S. Jung, M. A. Lauterbach, M. Bakhti, M. Gronborg, W. Mobius, J. Rhee, et al. (2010)
J. Cell Biol. 189, 223-232
   Abstract »    Full Text »    PDF »
The Connecdenn Family, Rab35 Guanine Nucleotide Exchange Factors Interfacing with the Clathrin Machinery.
A. L. Marat and P. S. McPherson (2010)
J. Biol. Chem. 285, 10627-10637
   Abstract »    Full Text »    PDF »
Rab35 Mediates Transport of Cdc42 and Rac1 to the Plasma Membrane during Phagocytosis.
J. Shim, S.-M. Lee, M. S. Lee, J. Yoon, H.-S. Kweon, and Y.-J. Kim (2010)
Mol. Cell. Biol. 30, 1421-1433
   Abstract »    Full Text »    PDF »
Asymmetric Microtubule Function Is an Essential Requirement for Polarized Organization of the Drosophila Bristle.
A. Bitan, G. M. Guild, D. Bar-Dubin, and U. Abdu (2010)
Mol. Cell. Biol. 30, 496-507
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882