Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 326 (5955): 994-998

Copyright © 2009 by the American Association for the Advancement of Science

Two Chemoreceptors Mediate Developmental Effects of Dauer Pheromone in C. elegans

Kyuhyung Kim,1 Koji Sato,2 Mayumi Shibuya,1 Danna M. Zeiger,1 Rebecca A. Butcher,3 Justin R. Ragains,3 Jon Clardy,3 Kazushige Touhara,2 Piali Sengupta1,*

Abstract: Intraspecific chemical communication is mediated by signals called pheromones. Caenorhabditis elegans secretes a mixture of small molecules (collectively termed dauer pheromone) that regulates entry into the alternate dauer larval stage and also modulates adult behavior via as yet unknown receptors. Here, we identify two heterotrimeric GTP-binding protein (G protein)–coupled receptors (GPCRs) that mediate dauer formation in response to a subset of dauer pheromone components. The SRBC-64 and SRBC-66 GPCRs are members of the large Caenorhabditis-specific SRBC subfamily and are expressed in the ASK chemosensory neurons, which are required for pheromone-induced dauer formation. Expression of both, but not each receptor alone, confers pheromone-mediated effects on heterologous cells. Identification of dauer pheromone receptors will allow a better understanding of the signaling cascades that transduce the context-dependent effects of ecologically important chemical signals.

1 Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA.
2 Department of Integrated Biosciences, University of Tokyo, Chiba 277-8562, Japan.
3 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: sengupta{at}

Cell- and subunit-specific mechanisms of CNG channel ciliary trafficking and localization in C. elegans.
M. Wojtyniak, A. G. Brear, D. M. O'Halloran, and P. Sengupta (2013)
J. Cell Sci. 126, 4381-4395
   Abstract »    Full Text »    PDF »
Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1.
A. H. Ludewig, Y. Izrayelit, D. Park, R. U. Malik, A. Zimmermann, P. Mahanti, B. W. Fox, A. Bethke, F. Doering, D. L. Riddle, et al. (2013)
PNAS 110, 5522-5527
   Abstract »    Full Text »    PDF »
Transmembrane protein OSTA-1 shapes sensory cilia morphology via regulation of intracellular membrane trafficking in C. elegans.
A. Olivier-Mason, M. Wojtyniak, R. V. Bowie, I. V. Nechipurenko, O. E. Blacque, and P. Sengupta (2013)
Development 140, 1560-1572
   Abstract »    Full Text »    PDF »
Long noncoding RNAs in C. elegans.
J.-W. Nam and D. P. Bartel (2012)
Genome Res. 22, 2529-2540
   Abstract »    Full Text »    PDF »
Oxytocin/Vasopressin-Related Peptides Have an Ancient Role in Reproductive Behavior.
J. L. Garrison, E. Z. Macosko, S. Bernstein, N. Pokala, D. R. Albrecht, and C. I. Bargmann (2012)
Science 338, 540-543
   Abstract »    Full Text »    PDF »
Interaction of structure-specific and promiscuous G-protein-coupled receptors mediates small-molecule signaling in Caenorhabditis elegans.
D. Park, I. O'Doherty, R. K. Somvanshi, A. Bethke, F. C. Schroeder, U. Kumar, and D. L. Riddle (2012)
PNAS 109, 9917-9922
   Abstract »    Full Text »    PDF »
Degeneracy and Neuromodulation among Thermosensory Neurons Contribute to Robust Thermosensory Behaviors in Caenorhabditis elegans.
M. Beverly, S. Anbil, and P. Sengupta (2011)
J. Neurosci. 31, 11718-11727
   Abstract »    Full Text »    PDF »
Specific insulin-like peptides encode sensory information to regulate distinct developmental processes.
A. Cornils, M. Gloeck, Z. Chen, Y. Zhang, and J. Alcedo (2011)
Development 138, 1183-1193
   Abstract »    Full Text »    PDF »
Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans.
E. A. Hallem, W. C. Spencer, R. D. McWhirter, G. Zeller, S. R. Henz, G. Ratsch, D. M. Miller III, H. R. Horvitz, P. W. Sternberg, and N. Ringstad (2011)
PNAS 108, 254-259
   Abstract »    Full Text »    PDF »
Olfactory Plasticity Is Regulated by Pheromonal Signaling in Caenorhabditis elegans.
K. Yamada, T. Hirotsu, M. Matsuki, R. A. Butcher, M. Tomioka, T. Ishihara, J. Clardy, H. Kunitomo, and Y. Iino (2010)
Science 329, 1647-1650
   Abstract »    Full Text »    PDF »
The homeodomain protein hmbx-1 maintains asymmetric gene expression in adult C. elegans olfactory neurons.
B. J. Lesch and C. I. Bargmann (2010)
Genes & Dev. 24, 1802-1815
   Abstract »    Full Text »    PDF »
Strategies to Get Arrested.
A. Ogawa and R. J. Sommer (2009)
Science 326, 944-945
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882