Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 326 (5958): 1406-1410

Copyright © 2009 by the American Association for the Advancement of Science

Planarian Hh Signaling Regulates Regeneration Polarity and Links Hh Pathway Evolution to Cilia

Jochen C. Rink,* Kyle A. Gurley,* Sarah A. Elliott, Alejandro Sánchez Alvarado{dagger}

Abstract: The Hedgehog (Hh) signaling pathway plays multiple essential roles during metazoan development, homeostasis, and disease. Although core protein components are highly conserved, the variations in Hh signal transduction mechanisms exhibited by existing model systems (Drosophila, fish, and mammals) are difficult to understand. We characterized the Hh pathway in planarians. Hh signaling is essential for establishing the anterior/posterior axis during regeneration by modulating wnt expression. Moreover, RNA interference methods to reduce signal transduction proteins Cos2/Kif27/Kif7, Fused, or Iguana do not result in detectable Hh signaling defects; however, these proteins are essential for planarian ciliogenesis. Our study expands the understanding of Hh signaling in the animal kingdom and suggests an ancestral mechanistic link between Hh signaling and the function of cilia.

Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah School of Medicine, 401 MREB, 20 North 1900 East, Salt Lake City, UT 84103, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: sanchez{at}neuro.utah.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Towards a bioinformatics of patterning: a computational approach to understanding regulative morphogenesis.
D. Lobo, T. J. Malone, and M. Levin (2013)
Biology Open 2, 156-169
   Abstract »    Full Text »    PDF »
PBX/extradenticle is required to re-establish axial structures and polarity during planarian regeneration.
R. A. Blassberg, D. A. Felix, B. Tejada-Romero, and A. A. Aboobaker (2013)
Development 140, 730-739
   Abstract »    Full Text »    PDF »
pbx is required for pole and eye regeneration in planarians.
C.-C. G. Chen, I. E. Wang, and P. W. Reddien (2013)
Development 140, 719-729
   Abstract »    Full Text »    PDF »
Follistatin antagonizes Activin signaling and acts with Notum to direct planarian head regeneration.
R. H. Roberts-Galbraith and P. A. Newmark (2013)
PNAS 110, 1363-1368
   Abstract »    Full Text »    PDF »
The Role of Apoptosis-Induced Proliferation for Regeneration and Cancer.
H. D. Ryoo and A. Bergmann (2012)
Cold Spring Harb Perspect Biol 4, a008797
   Abstract »    Full Text »    PDF »
Centrosome Loss in the Evolution of Planarians.
J. Azimzadeh, M. L. Wong, D. M. Downhour, A. S. Alvarado, and W. F. Marshall (2012)
Science 335, 461-463
   Abstract »    Full Text »    PDF »
Congenital Hydrocephalus in Genetically Engineered Mice.
P. Vogel, R. W. Read, G. M. Hansen, B. J. Payne, D. Small, A. T. Sands, and B. P. Zambrowicz (2012)
Veterinary Pathology 49, 166-181
   Abstract »    Full Text »    PDF »
Opposing Roles of Voltage-Gated Ca2+ Channels in Neuronal Control of Regenerative Patterning.
D. Zhang, J. D. Chan, T. Nogi, and J. S. Marchant (2011)
J. Neurosci. 31, 15983-15995
   Abstract »    Full Text »    PDF »
A LIM-homeobox gene is required for differentiation of Wnt-expressing cells at the posterior end of the planarian body.
T. Hayashi, M. Motoishi, S. Yazawa, K. Itomi, C. Tanegashima, O. Nishimura, K. Agata, and H. Tarui (2011)
Development 138, 3679-3688
   Abstract »    Full Text »    PDF »
The maintenance and regeneration of the planarian excretory system are regulated by EGFR signaling.
J. C. Rink, H. T.-K. Vu, and A. S. Alvarado (2011)
Development 138, 3769-3780
   Abstract »    Full Text »    PDF »
Alejandro Sanchez Alvarado: Bootstrapping flatworms into the molecular age.
C. Sedwick (2011)
J. Cell Biol. 194, 510-511
   Full Text »    PDF »
Polarized notum Activation at Wounds Inhibits Wnt Function to Promote Planarian Head Regeneration.
C. P. Petersen and P. W. Reddien (2011)
Science 332, 852-855
   Abstract »    Full Text »    PDF »
Cilia-driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear.
X. Yu, D. Lau, C. P. Ng, and S. Roy (2011)
Development 138, 487-494
   Abstract »    Full Text »    PDF »
The planarian flatworm: an in vivo model for stem cell biology and nervous system regeneration.
L. Gentile, F. Cebria, and K. Bartscherer (2011)
Dis. Model. Mech. 4, 12-19
   Abstract »    Full Text »    PDF »
An Outer Arm Dynein Conformational Switch Is Required for Metachronal Synchrony of Motile Cilia in Planaria.
P. Rompolas, R. S. Patel-King, and S. M. King (2010)
Mol. Biol. Cell 21, 3669-3679
   Abstract »    Full Text »    PDF »
Apoptosis, Stem Cells, and Tissue Regeneration.
A. Bergmann and H. Steller (2010)
Science Signaling 3, re8
   Abstract »    Full Text »    PDF »
Mechanism and evolution of cytosolic Hedgehog signal transduction.
C. W. Wilson and P.-T. Chuang (2010)
Development 137, 2079-2094
   Abstract »    Full Text »    PDF »
Wnt Signaling in Axial Patterning and Regeneration: Lessons from Planaria.
E. M. De Robertis (2010)
Science Signaling 3, pe21
   Abstract »    Full Text »    PDF »
Cell migration that orients the dorsoventral axis is coordinated with anteroposterior patterning mediated by Hedgehog signaling in the early spider embryo.
Y. Akiyama-Oda and H. Oda (2010)
Development 137, 1263-1273
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882