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Science 327 (5967): 866-868

Copyright © 2010 by the American Association for the Advancement of Science

Vibrio cholerae VpsT Regulates Matrix Production and Motility by Directly Sensing Cyclic di-GMP

Petya V. Krasteva,1 Jiunn C. N. Fong,2 Nicholas J. Shikuma,2 Sinem Beyhan,2 Marcos V. A. S. Navarro,1 Fitnat H. Yildiz,2,* Holger Sondermann1,*

Abstract: Microorganisms can switch from a planktonic, free-swimming life-style to a sessile, colonial state, called a biofilm, which confers resistance to environmental stress. Conversion between the motile and biofilm life-styles has been attributed to increased levels of the prokaryotic second messenger cyclic di-guanosine monophosphate (c-di-GMP), yet the signaling mechanisms mediating such a global switch are poorly understood. Here we show that the transcriptional regulator VpsT from Vibrio cholerae directly senses c-di-GMP to inversely control extracellular matrix production and motility, which identifies VpsT as a master regulator for biofilm formation. Rather than being regulated by phosphorylation, VpsT undergoes a change in oligomerization on c-di-GMP binding.

1 Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
2 Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, CA 95064, USA.

* To whom correspondence should be addressed: yildiz{at}metx.ucsc.edu (F.H.Y.); hs293{at}cornell.edu (H.S.)

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