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Science 328 (5974): 85-89

Copyright © 2010 by the American Association for the Advancement of Science

Orchestration of Floral Initiation by APETALA1

Kerstin Kaufmann,1,2,* Frank Wellmer,3,* Jose M. Muiño,4 Thilia Ferrier,5 Samuel E. Wuest,3 Vijaya Kumar,6 Antonio Serrano-Mislata,7 Francisco Madueño,7 Pawel Krajewski,8 Elliot M. Meyerowitz,6 Gerco C. Angenent,1,9 José Luis Riechmann5,6,10,{dagger}

Abstract: The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using a combination of gene expression profiling and genome-wide binding studies. Many of its targets encode transcriptional regulators, including known floral repressors. The latter genes are down-regulated by AP1, suggesting that it initiates floral development by abrogating the inhibitory effects of these genes. Although AP1 acts predominantly as a transcriptional repressor during the earliest stages of flower development, at more advanced stages it also activates regulatory genes required for floral organ formation, indicating a dynamic mode of action. Our results further imply that AP1 orchestrates floral initiation by integrating growth, patterning, and hormonal pathways.

1 Business Unit Bioscience, Plant Research International, Wageningen 6700 AA, Netherlands.
2 Laboratory of Molecular Biology, Wageningen University, Wageningen 6700 AP, Netherlands.
3 Smurfit Institute of Genetics, Trinity College, Dublin 2, Ireland.
4 Applied Bioinformatics, Plant Research International, Wageningen 6700 AA, Netherlands.
5 Center for Research in Agricultural Genomics (CRAG), Barcelona 08034, Spain.
6 California Institute of Technology, Division of Biology, Pasadena, CA 91125, USA.
7 Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas–Universidad Politécnica de Valencia, Valencia 46022, Spain.
8 Institute of Plant Genetics, Polish Academy of Sciences, Poznan 60-479, Poland.
9 Centre for BioSystems Genomics (CBSG), Wageningen 6700 AB, Netherlands.
10 Institució Catalana de Recerca i Estudis Avançats, Barcelona 08010, Spain.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: jriechma{at}caltech.edu


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