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Science 329 (5994): 956-959

Copyright © 2010 by the American Association for the Advancement of Science

Linear Arrays of Nuclear Envelope Proteins Harness Retrograde Actin Flow for Nuclear Movement

G. W. Gant Luxton,1,* Edgar R. Gomes,1,2,3,* Eric S. Folker,1 Erin Vintinner,1 Gregg G. Gundersen1,{dagger}

Abstract: Nuclei move to specific locations to polarize migrating and differentiating cells. Many nuclear movements are microtubule-dependent. However, nuclear movement to reorient the centrosome in migrating fibroblasts occurs through an unknown actin-dependent mechanism. We found that linear arrays of outer (nesprin2G) and inner (SUN2) nuclear membrane proteins assembled on and moved with retrogradely moving dorsal actin cables during nuclear movement in polarizing fibroblasts. Inhibition of nesprin2G, SUN2, or actin prevented nuclear movement and centrosome reorientation. The coupling of actin cables to the nuclear membrane for nuclear movement via specific membrane proteins indicates that, like plasma membrane integrins, nuclear membrane proteins assemble into actin-dependent arrays for force transduction.

1 Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
2 UMR S 787 INSERM, Université Pierre et Marie Curie Paris VI, 75634 Paris, France.
3 Groupe Hospitalier Pitié-Salpêtrière, Institut de Myologie, 75013 Paris, France.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: ggg1{at}columbia.edu

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