Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 329 (5999): 1660-1662

Copyright © 2010 by the American Association for the Advancement of Science

A Vibrio Effector Protein Is an Inositol Phosphatase and Disrupts Host Cell Membrane Integrity

Christopher A. Broberg, Lingling Zhang, Herman Gonzalez, Michelle A. Laskowski-Arce, Kim Orth*

Abstract: The marine bacterium Vibrio parahaemolyticus causes gastroenteritis in humans and encodes the type III effector protein VPA0450, which contributes to host cell death caused by autophagy, cell rounding, and cell lysis. We found that VPA0450 is an inositol polyphosphate 5-phosphatase that hydrolyzed the D5 phosphate from the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate. VPA0450 disrupted cytoskeletal binding sites on the inner surface of membranes of human cells and caused plasma membrane blebbing, which compromised membrane integrity and probably contributed to cell death by facilitating lysis. Thus, bacterial pathogens can disrupt adaptor protein–binding sites required for proper membrane and cytoskeleton dynamics by altering the homeostasis of membrane-bound inositol-signaling molecules.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

* To whom correspondence should be addressed. E-mail: kim.orth{at}

Loss of Sigma Factor RpoN Increases Intestinal Colonization of Vibrio parahaemolyticus in an Adult Mouse Model.
W. B. Whitaker, G. P. Richards, E. F. Boyd, and S. M. Payne (2014)
Infect. Immun. 82, 544-556
   Abstract »    Full Text »    PDF »
Phosphoinositide-mediated oligomerization of a defensin induces cell lysis.
I. K. Poon, A. A. Baxter, F. T. Lay, G. D. Mills, C. G. Adda, J. A. Payne, T. K. Phan, G. F. Ryan, J. A. White, P. K. Veneer, et al. (2014)
eLife Sci 3, e01808
   Abstract »    Full Text »    PDF »
Aeromonas salmonicida Ati2 is an effector protein of the type three secretion system.
S. Dallaire-Dufresne, X. Barbeau, D. Sarty, K. H. Tanaka, A. M. Denoncourt, P. Lague, M. E. Reith, and S. J. Charette (2013)
Microbiology 159, 1937-1945
   Abstract »    Full Text »    PDF »
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation.
T. Balla (2013)
Physiol Rev 93, 1019-1137
   Abstract »    Full Text »    PDF »
Vibrio parahaemolyticus ExsE is requisite for initial adhesion and subsequent type III secretion system 1-dependent autophagy in HeLa cells.
D. P. Erwin, S. D. Nydam, and D. R. Call (2012)
Microbiology 158, 2303-2314
   Abstract »    Full Text »    PDF »
Subversion of Cell Signaling by Pathogens.
N. M. Alto and K. Orth (2012)
Cold Spring Harb Perspect Biol 4, a006114
   Abstract »    Full Text »    PDF »
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model.
W. B. Whitaker, M. A. Parent, A. Boyd, G. P. Richards, and E. F. Boyd (2012)
Infect. Immun. 80, 1834-1845
   Abstract »    Full Text »    PDF »
Quorum Sensing and Silencing in Vibrio parahaemolyticus.
C. J. Gode-Potratz and L. L. McCarter (2011)
J. Bacteriol. 193, 4224-4237
   Abstract »    Full Text »    PDF »
Outer membrane adhesion factor multivalent adhesion molecule 7 initiates host cell binding during infection by Gram-negative pathogens.
A. M. Krachler, H. Ham, and K. Orth (2011)
PNAS 108, 11614-11619
   Abstract »    Full Text »    PDF »
Vibrio cholerae Requires the Type VI Secretion System Virulence Factor VasX To Kill Dictyostelium discoideum.
S. T. Miyata, M. Kitaoka, T. M. Brooks, S. B. McAuley, and S. Pukatzki (2011)
Infect. Immun. 79, 2941-2949
   Abstract »    Full Text »    PDF »
Bacterial Exploitation of Host Cell Signaling.
R. Lu and M. B. Goldberg (2010)
Science Translational Medicine 2, 51ps48
   Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882