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Pathogenomics of Culex quinquefasciatus and Meta-Analysis of Infection Responses to Diverse Pathogens
Lyric C. Bartholomay,1,*
Robert M. Waterhouse,2,3,15,*
George F. Mayhew,4
Corey L. Campbell,5
Kristin Michel,6
Zhen Zou,7
Jose L. Ramirez,8
Suchismita Das,8
Kanwal Alvarez,7
Peter Arensburger,9
Bart Bryant,6,7
Sinead B. Chapman,10
Yuemei Dong,8
Sara M. Erickson,4
S. H. P. Parakrama Karunaratne,11,12
Vladimir Kokoza,7
Chinnappa D. Kodira,13
Patricia Pignatelli,11
Sang Woon Shin,7
Dana L. Vanlandingham,14
Peter W. Atkinson,9
Bruce Birren,10
George K. Christophides,15
Rollie J. Clem,6
Janet Hemingway,11
Stephen Higgs,14
Karine Megy,16
Hilary Ranson,11
Evgeny M. Zdobnov,2,3,15
Alexander S. Raikhel,7
Bruce M. Christensen,4
George Dimopoulos,8
Marc A. T. Muskavitch10,17,18,
Abstract:
The mosquito Culex quinquefasciatus poses a substantial threatto human and veterinary health as a primary vector of West Nilevirus (WNV), the filarial worm Wuchereria bancrofti, and anavian malaria parasite. Comparative phylogenomics revealed anexpanded canonical C. quinquefasciatus immune gene repertoirecompared with those of Aedes aegypti and Anopheles gambiae.Transcriptomic analysis of C. quinquefasciatus genes responsiveto WNV, W. bancrofti, and non-native bacteria facilitated anunprecedented meta-analysis of 25 vector-pathogen interactionsinvolving arboviruses, filarial worms, bacteria, and malariaparasites, revealing common and distinct responses to thesepathogen types in three mosquito genera. Our findings providesupport for the hypothesis that mosquito-borne pathogens haveevolved to evade innate immune responses in three vector mosquitospecies of major medical importance.
1 Department of Entomology, Iowa State University, Ames, IA 50011, USA. 2 Department of Genetic Medicine and Development, University of Geneva Medical School, 1 Rue Michel-Servet, 1211 Geneva, CH, Switzerland. 3 Swiss Institute of Bioinformatics, 1 Rue Michel-Servet, 1211 Geneva, CH, Switzerland. 4 Department of Pathobiological Sciences, University of Wisconsin, Madison, WI 53706, USA. 5 Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523, USA. 6 Division of Biology, Arthropod Genomics Center, Molecular and Cellular Developmental Biology Program, Kansas State University, Manhattan, KS 66506, USA. 7 Department of Entomology, University of California, Riverside, CA 92521, USA. 8 W. Harry Feinstone Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA. 9 Department of Entomology, Center for Disease Vector Research, University of California, Riverside, CA 92521, USA. 10 The Broad Institute, Cambridge MA 02142, USA. 11 Liverpool School of Tropical Medicine, Liverpool, L3 5QA, UK. 12 Faculty of Science and Department of Zoology, University of Peradeniya, Peradeniya 20400, LK, Sri Lanka. 13 454 Life Sciences, Branford, CT 06405, USA. 14 Pathology Department, University of Texas Medical Branch, Galveston, TX 77555, USA. 15 Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK. 16 European Bioinformatics Institute (EMBL), Hinxton CB10 1SD Cambridge, UK. 17 Biology Department, Boston College, Chestnut Hill, MA 02467, USA. 18 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: marc.muskavitch{at}bc.edu
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In Science Magazine
REPORTS
Peter Arensburger, Karine Megy, Robert M. Waterhouse, Jenica Abrudan, Paolo Amedeo, Beatriz Antelo, Lyric Bartholomay, Shelby Bidwell, Elisabet Caler, Francisco Camara, Corey L. Campbell, Kathryn S. Campbell, Claudio Casola, Marta T. Castro, Ishwar Chandramouliswaran, Sinéad B. Chapman, Scott Christley, Javier Costas, Eric Eisenstadt, Cedric Feschotte, Claire Fraser-Liggett, Roderic Guigo, Brian Haas, Martin Hammond, Bill S. Hansson, Janet Hemingway, Sharon R. Hill, Clint Howarth, Rickard Ignell, Ryan C. Kennedy, Chinnappa D. Kodira, Neil F. Lobo, Chunhong Mao, George Mayhew, Kristin Michel, Akio Mori, Nannan Liu, Horacio Naveira, Vishvanath Nene, Nam Nguyen, Matthew D. Pearson, Ellen J. Pritham, Daniela Puiu, Yumin Qi, Hilary Ranson, Jose M. C. Ribeiro, Hugh M. Roberston, David W. Severson, Martin Shumway, Mario Stanke, Robert L. Strausberg, Cheng Sun, Granger Sutton, Zhijian (Jake) Tu, Jose Manuel C. Tubio, Maria F. Unger, Dana L. Vanlandingham, Albert J. Vilella, Owen White, Jared R. White, Charles S. Wondji, Jennifer Wortman, Evgeny M. Zdobnov, Bruce Birren, Bruce M. Christensen, Frank H. Collins, Anthony Cornel, George Dimopoulos, Linda I. Hannick, Stephen Higgs, Gregory C. Lanzaro, Daniel Lawson, Norman H. Lee, Marc A. T. Muskavitch, Alexander S. Raikhel, and Peter W. Atkinson (1 October 2010) Science330 (6000), 86.
[DOI: 10.1126/science.1191864] |Abstract »|Full Text »|PDF »|Supporting Online Material »
To whom correspondence should be addressed. E-mail: 
