Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 330 (6001): 235-239

Copyright © 2010 by the American Association for the Advancement of Science

Survivin Reads Phosphorylated Histone H3 Threonine 3 to Activate the Mitotic Kinase Aurora B

Alexander E. Kelly,1,*,{dagger} Cristina Ghenoiu,1,2,* John Z. Xue,1 Christian Zierhut,1 Hiroshi Kimura,3 Hironori Funabiki1,{dagger}

Abstract: A hallmark of mitosis is the appearance of high levels of histone phosphorylation, yet the roles of these modifications remain largely unknown. Here, we demonstrate that histone H3 phosphorylated at threonine 3 is directly recognized by an evolutionarily conserved binding pocket in the BIR domain of Survivin, which is a member of the chromosomal passenger complex (CPC). This binding mediates recruitment of the CPC to chromosomes and the resulting activation of its kinase subunit Aurora B. Consistently, modulation of the kinase activity of Haspin, which phosphorylates H3T3, leads to defects in the Aurora B–dependent processes of spindle assembly and inhibition of nuclear reformation. These findings establish a direct cellular role for mitotic histone H3T3 phosphorylation, which is read and translated by the CPC to ensure accurate cell division.

1 Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA.
2 Weill Cornell Graduate School of Biomedical Sciences, Department of Molecular Biology, Cornell Medical School, New York, NY 10021, USA.
3 School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: funabih{at}rockefeller.edu (H.F.); akelly{at}rockefeller.edu (A.E.K.)

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vitro Phosphorylation of Histone H3 at Threonine 3 by Arabidopsis Haspin Is Strongly Influenced by Posttranslational Modifications of Adjacent Amino Acids.
R. Karimi-Ashtiyani and A. Houben (2013)
Mol Plant 6, 574-576
   Full Text »    PDF »
The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly.
T. Hori, W.-H. Shang, K. Takeuchi, and T. Fukagawa (2013)
J. Cell Biol. 200, 45-60
   Abstract »    Full Text »    PDF »
Bub1 kinase activity drives error correction and mitotic checkpoint control but not tumor suppression.
R. M. Ricke, K. B. Jeganathan, L. Malureanu, A. M. Harrison, and J. M. van Deursen (2012)
J. Cell Biol. 199, 931-949
   Abstract »    Full Text »    PDF »
On the molecular mechanisms of mitotic kinase activation.
R. Bayliss, A. Fry, T. Haq, and S. Yeoh (2012)
Open Bio 2, 120136
   Abstract »    Full Text »    PDF »
Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation.
F. Wang, N. P. Ulyanova, J. R. Daum, D. Patnaik, A. V. Kateneva, G. J. Gorbsky, and J. M. G. Higgins (2012)
J. Cell Biol. 199, 251-268
   Abstract »    Full Text »    PDF »
A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
A. De Antoni, S. Maffini, S. Knapp, A. Musacchio, and S. Santaguida (2012)
J. Cell Biol. 199, 269-284
   Abstract »    Full Text »    PDF »
MLL5 maintains genomic integrity by regulating the stability of the chromosomal passenger complex through a functional interaction with Borealin.
J. Liu, F. Cheng, and L.-W. Deng (2012)
J. Cell Sci. 125, 4676-4685
   Abstract »    Full Text »    PDF »
Aurora B kinase phosphorylates and instigates degradation of p53.
C. P. Gully, G. Velazquez-Torres, J.-H. Shin, E. Fuentes-Mattei, E. Wang, C. Carlock, J. Chen, D. Rothenberg, H. P. Adams, H. H. Choi, et al. (2012)
PNAS 109, E1513-E1522
   Abstract »    Full Text »    PDF »
Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres.
E. Niedzialkowska, F. Wang, P. J. Porebski, W. Minor, J. M. G. Higgins, and P. T. Stukenberg (2012)
Mol. Biol. Cell 23, 1457-1466
   Abstract »    Full Text »    PDF »
Contrasting roles of condensin I and condensin II in mitotic chromosome formation.
L. C. Green, P. Kalitsis, T. M. Chang, M. Cipetic, J. H. Kim, O. Marshall, L. Turnbull, C. B. Whitchurch, P. Vagnarelli, K. Samejima, et al. (2012)
J. Cell Sci. 125, 1591-1604
   Abstract »    Full Text »    PDF »
PR-Set7 and H4K20me1: at the crossroads of genome integrity, cell cycle, chromosome condensation, and transcription.
D. B. Beck, H. Oda, S. S. Shen, and D. Reinberg (2012)
Genes & Dev. 26, 325-337
   Abstract »    Full Text »    PDF »
Spindle assembly checkpoint: the third decade.
A. Musacchio (2011)
Phil Trans R Soc B 366, 3595-3604
   Abstract »    Full Text »    PDF »
The Renaissance or the cuckoo clock.
J. Pines and I. Hagan (2011)
Phil Trans R Soc B 366, 3625-3634
   Abstract »    Full Text »    PDF »
Phosphorylation at serine 331 is required for Aurora B activation.
E. Petsalaki, T. Akoumianaki, E. J. Black, D. A. F. Gillespie, and G. Zachos (2011)
J. Cell Biol. 195, 449-466
   Abstract »    Full Text »    PDF »
Recognition of Unmodified Histone H3 by the First PHD Finger of Bromodomain-PHD Finger Protein 2 Provides Insights into the Regulation of Histone Acetyltransferases Monocytic Leukemic Zinc-finger Protein (MOZ) and MOZ-related factor (MORF).
S. Qin, L. Jin, J. Zhang, L. Liu, P. Ji, M. Wu, J. Wu, and Y. Shi (2011)
J. Biol. Chem. 286, 36944-36955
   Abstract »    Full Text »    PDF »
Role of Survivin in cytokinesis revealed by a separation-of-function allele.
E. Szafer-Glusman, M. T. Fuller, and M. G. Giansanti (2011)
Mol. Biol. Cell 22, 3779-3790
   Abstract »    Full Text »    PDF »
Expression patterns of Aurora A and B kinases, Ki-67 and the estrogen and progesterone receptors determined using an endometriosis tissue microarray model.
A. Calcagno, T. Grassi, L. Mariuzzi, S. Marzinotto, A. P. Londero, M. Orsaria, C. A. Beltrami, and D. Marchesoni (2011)
Hum. Reprod. 26, 2731-2741
   Abstract »    Full Text »    PDF »
Perturbation of Mitosis through Inhibition of Histone Acetyltransferases: The Key to Ochratoxin A Toxicity and Carcinogenicity?.
K. Czakai, K. Muller, P. Mosesso, G. Pepe, M. Schulze, A. Gohla, D. Patnaik, W. Dekant, J. M. G. Higgins, and A. Mally (2011)
Toxicol. Sci. 122, 317-329
   Abstract »    Full Text »    PDF »
Protein phosphatases and the regulation of mitosis.
F. A. Barr, P. R. Elliott, and U. Gruneberg (2011)
J. Cell Sci. 124, 2323-2334
   Abstract »    Full Text »    PDF »
Histone H3 phosphorylation and non-disjunction of the maternal X chromosome during male meiosis in sciarid flies.
M. C. Escriba, M. C. Giardini, and C. Goday (2011)
J. Cell Sci. 124, 1715-1725
   Abstract »    Full Text »    PDF »
Temporal and Spatial Regulation of Targeting Aurora B to the Inner Centromere.
Y. Watanabe (2011)
Cold Spring Harb Symp Quant Biol
   Abstract »    PDF »
Surfing Chromosomes (and Survivin).
A. Musacchio (2010)
Science 330, 183-184
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882