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Science 330 (6004): 665-669

Copyright © 2010 by the American Association for the Advancement of Science

Lineage Relationship Analysis of ROR{gamma}t+ Innate Lymphoid Cells

Shinichiro Sawa,1,2 Marie Cherrier,1,2 Matthias Lochner,1,2,3 Naoko Satoh-Takayama,4,5 Hans Jörg Fehling,6 Francina Langa,7 James P. Di Santo,4,5 Gérard Eberl1,2,*

Abstract: Lymphoid tissue–inducer (LTi) cells initiate the development of lymphoid tissues through the activation of local stromal cells in a process similar to inflammation. LTi cells express the nuclear hormone receptor ROR{gamma}t, which also directs the expression of the proinflammatory cytokine interleukin-17 in T cells. We show here that LTi cells are part of a larger family of proinflammatory ROR{gamma}t+ innate lymphoid cells (ILCs) that differentiate from distinct fetal liver ROR{gamma}t+ precursors. The fate of ROR{gamma}t+ ILCs is determined by mouse age, and after birth, favors the generation of cells involved in intestinal homeostasis and defense. Contrary to ROR{gamma}t+ T cells, however, ROR{gamma}t+ ILCs develop in the absence of microbiota. Our study indicates that ROR{gamma}t+ ILCs evolve to preempt intestinal colonization by microbial symbionts.

1 Lymphoid Tissue Development Unit, Institut Pasteur, 75724 Paris, France.
2 CNRS, URA1961, 75724 Paris, France.
3 Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
4 Innate Immunity Unit, Institut Pasteur, 75724 Paris, France.
5 Inserm, U668, 75724 Paris, France.
6 Institute of Immunology, University Clinics Ulm, Ulm, Germany.
7 Centre Ingénieurie Génétique Murine, Institut Pasteur, 75724 Paris, France.

* To whom correspondence should be addressed. E-mail: gerard.eberl{at}pasteur.fr


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