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Science 333 (6043): 769-773

Copyright © 2011 by the American Association for the Advancement of Science

Cartilage Acidic Protein–1B (LOTUS), an Endogenous Nogo Receptor Antagonist for Axon Tract Formation

Yasufumi Sato,1,* Masumi Iketani,1,* Yuji Kurihara,1 Megumi Yamaguchi,1 Naoya Yamashita,1 Fumio Nakamura,1 Yuko Arie,1 Takahiko Kawasaki,2 Tatsumi Hirata,2 Takaya Abe,3 Hiroshi Kiyonari,3 Stephen M. Strittmatter,4 Yoshio Goshima,1,5,{dagger} Kohtaro Takei1,5,{dagger}

Abstract: Neural circuitry formation depends on the molecular control of axonal projection during development. By screening with fluorophore-assisted light inactivation in the developing mouse brain, we identified cartilage acidic protein–1B as a key molecule for lateral olfactory tract (LOT) formation and named it LOT usher substance (LOTUS). We further identified Nogo receptor–1 (NgR1) as a LOTUS-binding protein. NgR1 is a receptor of myelin-derived axon growth inhibitors, such as Nogo, which prevent neural regeneration in the adult. LOTUS suppressed Nogo-NgR1 binding and Nogo-induced growth cone collapse. A defasciculated LOT was present in lotus-deficient mice but not in mice lacking both lotus- and ngr1. These findings suggest that endogenous antagonism of NgR1 by LOTUS is crucial for normal LOT formation.

1 Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.
2 Division of Brain Function, National Institute of Genetics, Graduate School for Advanced Studies, Mishima 411-8540, Japan.
3 Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
4 Department of Neurology and Section of Neurobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
5 Advanced Medical Research Center, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan.

* These authors contributed equally to this study.

{dagger} To whom correspondence should be addressed. E-mail: kohtaro{at}med.yokohama-cu.ac.jp (K.T.); goshima{at}med.yokohama-cu.ac.jp (Y.G.)


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