Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 333 (6046): 1144-1147

Copyright © 2011 by the American Association for the Advancement of Science

Nectins Establish a Checkerboard-Like Cellular Pattern in the Auditory Epithelium

Hideru Togashi,1,* Kanoko Kominami,1,* Masazumi Waseda,1,{dagger} Hitomi Komura,1,{ddagger} Jun Miyoshi,2 Masatoshi Takeichi,3 Yoshimi Takai1,§

Abstract: In the auditory epithelium of the cochlea, the sensory hair cells and supporting cells are arranged in a checkerboard-like fashion, but the mechanism underlying this cellular patterning is unclear. We found that mouse hair cells and supporting cells express the immunoglobulin-like adhesion molecules nectin-1 and -3, respectively, and that their interaction mediates the heterotypic adhesion between these two cell types. Genetic removal of nectin-1 or -3 disrupted the checkerboard-like pattern, inducing aberrant attachment between hair cells. When cells expressing either nectin-1 or -3 were cocultured, they arranged themselves into a mosaic pattern. Thus, nectin-1 and -3 promote the formation of the checkerboard-like pattern of the auditory epithelia.

1 Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
2 Department of Molecular Biology, Osaka Medical Center for Cancer and Cardiovascular Disease, Osaka 537-8511, Japan.
3 Laboratory for Cell Adhesion and Tissue Patterning, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.

* These authors contributed equally to this work.

{dagger} Present address: Division of Genetics and Department of Cancer Biology, the Institute of Medical Science, the University of Tokyo, Tokyo 108-8639, Japan.

{ddagger} Present address: Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

§ To whom correspondence should be addressed. E-mail: ytakai{at}med.kobe-u.ac.jp


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Aberrant cochlear hair cell attachments caused by Nectin-3 deficiency result in hair bundle abnormalities.
T. Fukuda, K. Kominami, S. Wang, H. Togashi, K.-i. Hirata, A. Mizoguchi, Y. Rikitake, and Y. Takai (2014)
Development 141, 399-409
   Abstract »    Full Text »    PDF »
Lis1 mediates planar polarity of auditory hair cells through regulation of microtubule organization.
C. W. Sipe, L. Liu, J. Lee, C. Grimsley-Myers, and X. Lu (2013)
Development 140, 1785-1795
   Abstract »    Full Text »    PDF »
Selective Ablation of Pillar and Deiters' Cells Severely Affects Cochlear Postnatal Development and Hearing in Mice.
M. M. Mellado Lagarde, B. C. Cox, J. Fang, R. Taylor, A. Forge, and J. Zuo (2013)
J. Neurosci. 33, 1564-1576
   Abstract »    Full Text »    PDF »
In vitro organogenesis in three dimensions: self-organising stem cells.
Y. Sasai, M. Eiraku, and H. Suga (2012)
Development 139, 4111-4121
   Abstract »    Full Text »    PDF »
Delta-like 1 and lateral inhibition during hair cell formation in the chicken inner ear: evidence against cis-inhibition.
E. Chrysostomou, J. E. Gale, and N. Daudet (2012)
Development 139, 3764-3774
   Abstract »    Full Text »    PDF »
Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion.
D. Samanta, U. A. Ramagopal, R. Rubinstein, V. Vigdorovich, S. G. Nathenson, and S. C. Almo (2012)
PNAS 109, 14836-14840
   Abstract »    Full Text »    PDF »
The role of nectins in different types of cell-cell adhesion.
Y. Rikitake, K. Mandai, and Y. Takai (2012)
J. Cell Sci. 125, 3713-3722
   Abstract »    Full Text »    PDF »
Crystal Structure of Cell Adhesion Molecule Nectin-2/CD112 and Its Binding to Immune Receptor DNAM-1/CD226.
J. Liu, X. Qian, Z. Chen, X. Xu, F. Gao, S. Zhang, R. Zhang, J. Qi, G. F. Gao, and J. Yan (2012)
J. Immunol. 188, 5511-5520
   Abstract »    Full Text »    PDF »
Structure of TIGIT immunoreceptor bound to poliovirus receptor reveals a cell-cell adhesion and signaling mechanism that requires cis-trans receptor clustering.
K. F. Stengel, K. Harden-Bowles, X. Yu, L. Rouge, J. Yin, L. Comps-Agrar, C. Wiesmann, J. F. Bazan, D. L. Eaton, and J. L. Grogan (2012)
PNAS 109, 5399-5404
   Abstract »    Full Text »    PDF »
Regulation of cochlear convergent extension by the vertebrate planar cell polarity pathway is dependent on p120-catenin.
M. F. Chacon-Heszele, D. Ren, A. B. Reynolds, F. Chi, and P. Chen (2012)
Development 139, 968-978
   Abstract »    Full Text »    PDF »
Arranging a Cellular Checkerboard.
W. Choi and M. Peifer (2011)
Science 333, 1099-1100
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882