Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 334 (6054): 362-365

Copyright © 2011 by the American Association for the Advancement of Science

Antimicrobial Peptides Keep Insect Endosymbionts Under Control

Frédéric H. Login,1,2 Séverine Balmand,1,2 Agnès Vallier,1,2 Carole Vincent-Monégat,1,2 Aurélien Vigneron,1,2 Michèle Weiss-Gayet,2,3 Didier Rochat,4 Abdelaziz Heddi1,2,*

Abstract: Vertically transmitted endosymbionts persist for millions of years in invertebrates and play an important role in animal evolution. However, the functional basis underlying the maintenance of these long-term resident bacteria is unknown. We report that the weevil coleoptericin-A (ColA) antimicrobial peptide selectively targets endosymbionts within the bacteriocytes and regulates their growth through the inhibition of cell division. Silencing the colA gene with RNA interference resulted in a decrease in size of the giant filamentous endosymbionts, which escaped from the bacteriocytes and spread into insect tissues. Although this family of peptides is commonly linked with microbe clearance, this work shows that endosymbiosis benefits from ColA, suggesting that long-term host-symbiont coevolution might have shaped immune effectors for symbiont maintenance.

1 INSA-Lyon, INRA, UMR203 BF2I, Biologie Fonctionnelle Insectes et Interactions, F-69621 Villeurbanne, France.
2 Université de Lyon, F-69003 Lyon, France.
3 Université Lyon 1, CNRS UMR5534, Centre de Génétique et de Physiologie Moléculaire et Cellulaire, F-69622 Villeurbanne, France.
4 INRA, Université Pierre et Marie Curie, UMR1272 Physiologie de l’Insecte Signalisation et Communication, F-78026 Versailles, France.

* To whom correspondence should be addressed. E-mail: abdelaziz.heddi{at}insa-lyon.fr


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Medicago truncatula symbiotic peptide NCR247 contributes to bacteroid differentiation through multiple mechanisms.
A. Farkas, G. Maroti, H. Durgő, Z. Gyorgypal, R. M. Lima, K. F. Medzihradszky, A. Kereszt, P. Mergaert, and E. Kondorosi (2014)
PNAS 111, 5183-5188
   Abstract »    Full Text »    PDF »
The Combined Effects of Bacterial Symbionts and Aging on Life History Traits in the Pea Aphid, Acyrthosiphon pisum.
A. M. Laughton, M. H. Fan, and N. M. Gerardo (2014)
Appl. Envir. Microbiol. 80, 470-477
   Abstract »    Full Text »    PDF »
Genome Degeneration and Adaptation in a Nascent Stage of Symbiosis.
K. F. Oakeson, R. Gil, A. L. Clayton, D. M. Dunn, A. C. von Niederhausern, C. Hamil, A. Aoyagi, B. Duval, A. Baca, F. J. Silva, et al. (2014)
Genome Biol Evol 6, 76-93
   Abstract »    Full Text »    PDF »
Specific Midgut Region Controlling the Symbiont Population in an Insect-Microbe Gut Symbiotic Association.
J. K. Kim, N. H. Kim, H. A. Jang, Y. Kikuchi, C.-H. Kim, T. Fukatsu, and B. L. Lee (2013)
Appl. Envir. Microbiol. 79, 7229-7233
   Abstract »    Full Text »    PDF »
Distinct antimicrobial peptide expression determines host species-specific bacterial associations.
S. Franzenburg, J. Walter, S. Kunzel, J. Wang, J. F. Baines, T. C. G. Bosch, and S. Fraune (2013)
PNAS 110, E3730-E3738
   Abstract »    Full Text »    PDF »
Bacterial Cell Wall Synthesis Gene uppP Is Required for Burkholderia Colonization of the Stinkbug Gut.
J. K. Kim, H. J. Lee, Y. Kikuchi, W. Kitagawa, N. Nikoh, T. Fukatsu, and B. L. Lee (2013)
Appl. Envir. Microbiol. 79, 4879-4886
   Abstract »    Full Text »    PDF »
Polyester synthesis genes associated with stress resistance are involved in an insect-bacterium symbiosis.
J. K. Kim, Y. J. Won, N. Nikoh, H. Nakayama, S. H. Han, Y. Kikuchi, Y. H. Rhee, H. Y. Park, J. Y. Kwon, K. Kurokawa, et al. (2013)
PNAS 110, E2381-E2389
   Abstract »    Full Text »    PDF »
Surveying the Microbiome of Ants: Comparing 454 Pyrosequencing with Traditional Methods To Uncover Bacterial Diversity.
S. Kautz, B. E. R. Rubin, J. A. Russell, and C. S. Moreau (2013)
Appl. Envir. Microbiol. 79, 525-534
   Abstract »    Full Text »    PDF »
Phage loss and the breakdown of a defensive symbiosis in aphids.
S. R. Weldon, M. R. Strand, and K. M. Oliver (2012)
Proc R Soc B 280, 20122103
   Abstract »    Full Text »    PDF »
Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont.
S. Shigenobu and D. L. Stern (2012)
Proc R Soc B 280, 20121952
   Abstract »    Full Text »    PDF »
Expansion of the antimicrobial peptide repertoire in the invasive ladybird Harmonia axyridis.
A. Vilcinskas, K. Mukherjee, and H. Vogel (2012)
Proc R Soc B 280, 20122113
   Abstract »    Full Text »    PDF »
Strategies of Genomic Integration Within Insect-Bacterial Mutualisms.
J. J. Wernegreen (2012)
Biol. Bull. 223, 112-122
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882