Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 334 (6059): 1137-1141

Copyright © 2011 by the American Association for the Advancement of Science

Polarization of PAR Proteins by Advective Triggering of a Pattern-Forming System

Nathan W. Goehring,1 Philipp Khuc Trong,2,1,* Justin S. Bois,2,1,{dagger} Debanjan Chowdhury,2,{ddagger} Ernesto M. Nicola,2,§ Anthony A. Hyman,1 Stephan W. Grill2,1,||

Abstract: In the Caenorhabditis elegans zygote, a conserved network of partitioning-defective (PAR) polarity proteins segregates into an anterior and a posterior domain, facilitated by flows of the cortical actomyosin meshwork. The physical mechanisms by which stable asymmetric PAR distributions arise from transient cortical flows remain unclear. We present evidence that PAR polarity arises from coupling of advective transport by the flowing cell cortex to a multistable PAR reaction-diffusion system. By inducing transient PAR segregation, advection serves as a mechanical trigger for the formation of a PAR pattern within an otherwise stably unpolarized system. We suggest that passive advective transport in an active and flowing material may be a general mechanism for mechanochemical pattern formation in developmental systems.

1 Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany.
2 Max Planck Institute for the Physics of Complex Systems (MPI-PKS), 01187 Dresden, Germany.

* Present address: Department of Physics, and Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge CB3 OHE, UK.

{dagger} Present address: Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.

{ddagger} Present address: Department of Physics, Harvard University, Cambridge, MA 02138, USA.

§ Present address: Institute for Cross-Disciplinary Physics and Complex Systems (IFISC), Consejo Superior de Investigaciones Cienti?cas–Universitat de les Illes Balears, E-07122 Palma de Mallorca, Spain.

||To whom correspondence should be addressed. E-mail: grill{at}mpi-cbg.de


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42.
S. E. Smith, B. Rubinstein, I. Mendes Pinto, B. D. Slaughter, J. R. Unruh, and R. Li (2013)
J. Cell Biol. 202, 1091-1106
   Abstract »    Full Text »    PDF »
The PAR network: redundancy and robustness in a symmetry-breaking system.
F. Motegi and G. Seydoux (2013)
Phil Trans R Soc B 368, 20130010
   Abstract »    Full Text »    PDF »
Cytoplasmic streaming in plant cells emerges naturally by microfilament self-organization.
F. G. Woodhouse and R. E. Goldstein (2013)
PNAS 110, 14132-14137
   Abstract »    Full Text »    PDF »
Mechanics of Epithelial Tissue Homeostasis and Morphogenesis.
C. Guillot and T. Lecuit (2013)
Science 340, 1185-1189
   Abstract »    Full Text »    PDF »
PAR-2, LGL-1 and the CDC-42 GAP CHIN-1 act in distinct pathways to maintain polarity in the C. elegans embryo.
A. Beatty, D. G. Morton, and K. Kemphues (2013)
Development 140, 2005-2014
   Abstract »    Full Text »    PDF »
Pattern formation of Rho GTPases in single cell wound healing.
C. M. Simon, E. M. Vaughan, W. M. Bement, and L. Edelstein-Keshet (2013)
Mol. Biol. Cell 24, 421-432
   Abstract »    Full Text »    PDF »
Cadherin-based intercellular adhesions organize epithelial cell-matrix traction forces.
A. F. Mertz, Y. Che, S. Banerjee, J. M. Goldstein, K. A. Rosowski, S. F. Revilla, C. M. Niessen, M. C. Marchetti, E. R. Dufresne, and V. Horsley (2013)
PNAS 110, 842-847
   Abstract »    Full Text »    PDF »
Cell polarity: models and mechanisms from yeast, worms and flies.
B. J. Thompson (2013)
Development 140, 13-21
   Abstract »    Full Text »    PDF »
Forces Driving Epithelial Spreading in Zebrafish Gastrulation.
M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch, S. W. Grill, and C.-P. Heisenberg (2012)
Science 338, 257-260
   Abstract »    Full Text »    PDF »
Cell Polarity: Quantitative Modeling as a Tool in Cell Biology.
A. Mogilner, J. Allard, and R. Wollman (2012)
Science 336, 175-179
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882