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Science 339 (6123): 1071-1074

Copyright © 2013 by the American Association for the Advancement of Science

Mps1 and Ipl1/Aurora B Act Sequentially to Correctly Orient Chromosomes on the Meiotic Spindle of Budding Yeast

Régis E. Meyer,1 Seoyoung Kim,1,2,* David Obeso,1,2,{dagger} Paul D. Straight,3,{ddagger} Mark Winey,3 Dean S. Dawson1,2,§

Abstract: The conserved kinases Mps1 and Ipl1/Aurora B are critical for enabling chromosomes to attach to microtubules so that partner chromosomes will be segregated correctly from each other, but the precise roles of these kinases have been unclear. We imaged live yeast cells to elucidate the stages of chromosome-microtubule interactions and their regulation by Ipl1 and Mps1 through meiosis I. Ipl1 was found to release kinetochore-microtubule (kMT) associations after meiotic entry, liberating chromosomes to begin homologous pairing. Surprisingly, most chromosome pairs began their spindle interactions with incorrect kMT attachments. Ipl1 released these improper connections, whereas Mps1 triggered the formation of new force-generating microtubule attachments. This microtubule release and reattachment cycle could prevent catastrophic chromosome segregation errors in meiosis.

1 Department of Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA.
2 Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
3 Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309–0347, USA.

* Present address: Molecular Biology Program, Memorial Sloan-Kettering, 1275 York Avenue, New York, NY 10065, USA.

{dagger} Present address: Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA.

{ddagger} Present address: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843–2128, USA.

§ To whom correspondence should be addressed. E-mail: dawsond{at}omrf.org


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Chromosome Segregation in Budding Yeast: Sister Chromatid Cohesion and Related Mechanisms.
A. L. Marston (2014)
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Dual mechanisms prevent premature chromosome segregation during meiosis.
S. Kim, R. Meyer, H. Chuong, and D. S. Dawson (2013)
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