Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 339 (6126): 1445-1448

Copyright © 2013 by the American Association for the Advancement of Science

A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro

Shukry J. Habib,1,2,* Bi-Chang Chen,2 Feng-Chiao Tsai,3 Konstantinos Anastassiadis,4 Tobias Meyer,3 Eric Betzig,2 Roel Nusse1,*

Abstract: Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt–β-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear β-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.

1 Department of Developmental Biology, Howard Hughes Medical Institute, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 265 Campus Drive, Stanford, CA 94305, USA.
2 Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA.
3 Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.
4 BIOTEC, Technische Universität Dresden Tatzberg 47-51, 01307 Dresden, Germany.

* Corresponding author. E-mail: rnusse{at}stanford.edu (R.N.); shabib{at}stanford.edu (S.J.H.)


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CKD-Induced Wingless/Integration1 Inhibitors and Phosphorus Cause the CKD-Mineral and Bone Disorder.
Y. Fang, C. Ginsberg, M. Seifert, O. Agapova, T. Sugatani, T. C. Register, B. I. Freedman, M.-C. Monier-Faugere, H. Malluche, and K. A. Hruska (2014)
J. Am. Soc. Nephrol.
   Abstract »
Brain tumor specifies intermediate progenitor cell identity by attenuating {beta}-catenin/Armadillo activity.
H. Komori, Q. Xiao, B. M. McCartney, and C.-Y. Lee (2014)
Development 141, 51-62
   Abstract »    Full Text »    PDF »
Constructing stem cell microenvironments using bioengineering approaches.
D. A. Brafman (2013)
Physiol Genomics 45, 1123-1135
   Abstract »    Full Text »    PDF »
Porcn-dependent Wnt signaling is not required prior to mouse gastrulation.
S. Biechele, K. Cockburn, F. Lanner, B. J. Cox, and J. Rossant (2013)
Development 140, 2961-2971
   Abstract »    Full Text »    PDF »
Making a Point with Wnt Signals.
J. D. Berndt and R. T. Moon (2013)
Science 339, 1388-1389
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882