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Science 339 (6127): 1625-1629

Copyright © 2013 by the American Association for the Advancement of Science

ESCRT-III Assembly and Cytokinetic Abscission Are Induced by Tension Release in the Intercellular Bridge

Julie Lafaurie-Janvore,1 Paolo Maiuri,1 Irène Wang,2 Mathieu Pinot,1 Jean-Baptiste Manneville,1 Timo Betz,3 Martial Balland,2 Matthieu Piel1,*

Abstract: The last step of cell division, cytokinesis, produces two daughter cells that remain connected by an intercellular bridge. This state often represents the longest stage of the division process. Severing the bridge (abscission) requires a well-described series of molecular events, but the trigger for abscission remains unknown. We found that pulling forces exerted by daughter cells on the intercellular bridge appear to regulate abscission. Counterintuitively, these forces prolonged connection, whereas a release of tension induced abscission. Tension release triggered the assembly of ESCRT-III (endosomal sorting complex required for transport–III), which was followed by membrane fission. This mechanism may allow daughter cells to remain connected until they have settled in their final locations, a process potentially important for tissue organization and morphogenesis.

1 Institut Curie, CNRS UMR 144, 26 rue d’Ulm, 75248 Paris Cedex 05, France.
2 Université de Grenoble 1/CNRS, LIPhy UMR 5588, Grenoble F-38041, France.
3 Institut Curie, CNRS UMR 168, 26 rue d’Ulm, 75248 Paris Cedex 05, France.

* Corresponding author. E-mail: matthieu.piel{at}curie.fr


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