Sci. Signal., 27 May 2008
Cell Migration Degrading Polyamines for Migration
Annalisa M. VanHook
Science Signaling, AAAS, Washington, DC 20005, USA
Integrins are heterodimeric transmembrane proteins that mediate cell adhesion and migration through various mechanisms. Binding of the cytoplasmic domain of the integrin 9 subunit to the spermidine/spermine-N1-acetyltransferase (SSAT)--which induces the breakdown of the higher-order polyamines spermidine and spermine to the lower-order polyamine putrescine--is required for migration mediated by the 9β1 integrin. deHart et al. (see commentary by Vandenberg) report that 9 recruitment of SSAT to focal adhesions affects cell migration by modulating the activity of an inward rectifier K+ (Kir) channel. Both wild-type and catalytically inactive forms of in vitro-translated SSAT bound to tagged 9 in a pull-down assay. Expression of any of the catalytically inactive forms in 9-expressing Chinese hamster ovary (CHO) cells prevented migration on an 9-specific ligand in a dominant-negative fashion. This effect was specific to 9, because migration mediated by a chimeric 95 protein, in which the intracellular domain of 9 was replaced with the intracellular domain of 5, was not affected by expression of the catalytically inactive SSATs. Depleting intracellular spermidine concentrations, using a combination of the ornithine decarboxylase inhibitor DFMO and putrescine, inhibited migration of CHO cells that overexpressed wild-type 9 but not those expressing the 95 chimera. Unexpectedly, restoring spermidine concentration by addition of putrescine in DFMO-treated cells restored migration. Furthermore, in mouse embryo fibroblasts (MEFs) overexpressing 9, but not those overexpressing the chimeric 95, migration was reduced when the expression of polyamine oxidase (PAO), an enzyme required for polyamine metabolism downstream of SSAT, was knocked down by small interfering RNAs (siRNAs). Adding putrescine did not restore migration to the PAO-deficient cells. Thus, the results from experiments in which global intracellular polyamine concentrations were altered with pharmacological agents were complex but may indicate that local changes in cellular polyamine concentrations at the focal adhesion regulate migration. K+ efflux is implicated in cell migration, and Kir channels are inhibited by spermidine and spermine; thus, SSAT may modulate polyamine concentrations to stimulate migration. Indeed, treating 9- (but not 94- or 95-) expressing MEFs with Ba2+ to inhibit Kir channels or with a 9β1-blocking antibody decreased migration of cells on an 9-specific ligand but had no effect on adhesion of cells to nonintegrin-specific substrates. Of the eight Kir subunits detected in MEF cells, only Kir4.2 appeared to have a role in 9-mediated migration. Knockdown of Kir4.2 by a short hairpin RNA (shRNA) or inhibition of Kir channels with Ba2+ in either 9-expressing MEFs or human microvascular endothelial cells, which natively express Kir4.2 and 9β1, impaired migration on 9 substrates. In the MEFs expressing 9, the effects of DFMO and Kir4.2 shRNA treatment were not additive, suggesting that polyamines regulate cell migration through Kir4.2. In an in vitro wound assay, 9-expressing MEF cells treated with Ba2+, Kir4.2 shRNA, or both migrated shorter distances than untreated cells and extended multiple leading-edge cytoplasmic projections, whereas untreated cells usually extended only one lamellopodium. Fluorescently tagged 9 and Kir4.2 colocalized to structures that looked like focal adhesions, but only at the leading edge of MEFs. These results suggest that the 9 subunit of integrins recruits SSAT to focal adhesions, where it decreases the local concentration of higher-order polyamines; this may relieve repression of Kir channels, thus initiating an outward K+ flow. The authors model that the K+ efflux may cause the influx of Ca2+, which is known to stimulate migration in other contexts.
G. W. deHart, T. Jin, D. E. McCloskey, A. E. Pegg, D. Sheppard, The 9β1 integrin enhances cell migration by polyamine-mediated modulation of an inward-rectifier potassium channel. Proc. Natl. Acad. Sci. U.S.A. 105, 7188-7193 (2008). [Abstract] [Full Text]
C. A. Vandenberg, Integrins step up the pace of cell migration through polyamines and potassium channels. Proc. Natl. Acad. Sci. U.S.A. 105, 7109-7110 (2008). [Full Text]
Citation: A. M. VanHook, Degrading Polyamines for Migration. Sci. Signal. 1, ec196 (2008).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882