Sci. Signal., 12 February 2008
Molecular Biology Specifying Gene Expression
Beverly A. Purnell
Science, AAAS, Washington, DC 20005, USA
The efficiency of gene transcription by RNA polymerase II is modulated by the composition of promoter chromatin, including the presence of nucleosome-binding proteins. Using a genomic approach, Krishnakumar et al. show that poly(ADP-ribose) polymerase-1 (PARP-1) and the linker histone H1, two nucleosome-binding proteins that compete for binding to nucleosomes in vitro, exhibit a reciprocal pattern of binding at many promoters in vivo. At positively regulated promoters, PARP-1 is bound but H1 is not. Thus, promoter localization patterns of nucleosome-binding factors can specify global gene expression outcomes in vivo.
R. Krishnakumar, M. J. Gamble, K. M. Frizzell, J. G. Berrocal, M. Kininis, W. L. Kraus, Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. Science 319, 819-821 (2008). [Abstract] [Full Text]
Citation: B. A. Purnell, Specifying Gene Expression. Sci. Signal. 1, ec59 (2008).
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