Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 12 February 2008
Vol. 1, Issue 6, p. ec59
[DOI: 10.1126/stke.16ec59]

EDITORS' CHOICE

Molecular Biology Specifying Gene Expression

Beverly A. Purnell

Science, AAAS, Washington, DC 20005, USA

The efficiency of gene transcription by RNA polymerase II is modulated by the composition of promoter chromatin, including the presence of nucleosome-binding proteins. Using a genomic approach, Krishnakumar et al. show that poly(ADP-ribose) polymerase-1 (PARP-1) and the linker histone H1, two nucleosome-binding proteins that compete for binding to nucleosomes in vitro, exhibit a reciprocal pattern of binding at many promoters in vivo. At positively regulated promoters, PARP-1 is bound but H1 is not. Thus, promoter localization patterns of nucleosome-binding factors can specify global gene expression outcomes in vivo.

R. Krishnakumar, M. J. Gamble, K. M. Frizzell, J. G. Berrocal, M. Kininis, W. L. Kraus, Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. Science 319, 819-821 (2008). [Abstract] [Full Text]

Citation: B. A. Purnell, Specifying Gene Expression. Sci. Signal. 1, ec59 (2008).



To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882