Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 20 January 2009
Vol. 2, Issue 54, p. pl1
[DOI: 10.1126/scisignal.254pl1]


Genome to Kinome: Species-Specific Peptide Arrays for Kinome Analysis

Shakiba Jalal1,2, Ryan Arsenault1,2, Andrew A. Potter1, Lorne A. Babiuk3, Philip J. Griebel1, and Scott Napper1*

1 Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada.
2 Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada.
3 University of Alberta, Edmonton, Alberta T6G 2J9, Canada.

Abstract: Tools for conducting high-throughput kinome analysis do not exist for many species. For example, two commonly used techniques for monitoring phosphorylation events are phosphorylation-specific antibodies and peptide arrays. The majority of phosphorylation-specific antibodies are for human or mouse targets, and the construction of peptide arrays relies on information from phosphorylation databases, which are similarly biased toward human and mouse data. This is a substantial obstacle because many species other than mouse represent important biological models. On the basis of the observation that phosphorylation events are often conserved across species with respect to their relative positioning within proteins and their biological function, we demonstrate that it is possible to predict the sequence contexts of phosphorylation events in other species for the production of peptide arrays for kinome analysis. Through this approach, genomic information can be rapidly used to create inexpensive, customizable, species-specific peptide arrays for high-throughput kinome analysis. We anticipate that these arrays will be valuable for investigating the conservation of biological responses across species, validating animal models of disease, and translating research to clinical applications.

* Corresponding author. E-mail, scott.napper{at}

Citation: S. Jalal, R. Arsenault, A. A. Potter, L. A. Babiuk, P. J. Griebel, S. Napper, Genome to Kinome: Species-Specific Peptide Arrays for Kinome Analysis. Sci. Signal. 2, pl1 (2009).

Read the Full Text

In Vitro Infection of Bovine Monocytes with Mycoplasma bovis Delays Apoptosis and Suppresses Production of Gamma Interferon and Tumor Necrosis Factor Alpha but Not Interleukin-10.
M. Mulongo, T. Prysliak, E. Scruten, S. Napper, and J. Perez-Casal (2014)
Infect. Immun. 82, 62-71
   Abstract »    Full Text »    PDF »
Divergent Immune Responses to Mycobacterium avium subsp. paratuberculosis Infection Correlate with Kinome Responses at the Site of Intestinal Infection.
P. Maattanen, B. Trost, E. Scruten, A. Potter, A. Kusalik, P. Griebel, and S. Napper (2013)
Infect. Immun. 81, 2861-2872
   Abstract »    Full Text »    PDF »
DAPPLE: a pipeline for the homology-based prediction of phosphorylation sites.
B. Trost, R. Arsenault, P. Griebel, S. Napper, and A. Kusalik (2013)
Bioinformatics 29, 1693-1695
   Abstract »    Full Text »    PDF »
cGMP-dependent protein kinase I{beta} regulates breast cancer cell migration and invasion via interaction with the actin/myosin-associated protein caldesmon.
R. Schwappacher, H. Rangaswami, J. Su-Yuo, A. Hassad, R. Spitler, and D. E. Casteel (2013)
J. Cell Sci. 126, 1626-1636
   Abstract »    Full Text »    PDF »
Altered Toll-Like Receptor 9 Signaling in Mycobacterium avium subsp. paratuberculosis-Infected Bovine Monocytes Reveals Potential Therapeutic Targets.
R. J. Arsenault, Y. Li, P. Maattanen, E. Scruten, K. Doig, A. Potter, P. Griebel, A. Kusalik, and S. Napper (2013)
Infect. Immun. 81, 226-237
   Abstract »    Full Text »    PDF »
Mycobacterium avium subsp. paratuberculosis Inhibits Gamma Interferon-Induced Signaling in Bovine Monocytes: Insights into the Cellular Mechanisms of Johne's Disease.
R. J. Arsenault, Y. Li, K. Bell, K. Doig, A. Potter, P. J. Griebel, A. Kusalik, and S. Napper (2012)
Infect. Immun. 80, 3039-3048
   Abstract »    Full Text »    PDF »
Systems Kinomics Demonstrates Congo Basin Monkeypox Virus Infection Selectively Modulates Host Cell Signaling Responses as Compared to West African Monkeypox Virus.
J. Kindrachuk, R. Arsenault, A. Kusalik, K. N. Kindrachuk, B. Trost, S. Napper, P. B. Jahrling, and J. E. Blaney (2012)
Mol. Cell. Proteomics 11, M111.015701
   Abstract »    Full Text »    PDF »
A Systematic Approach for Analysis of Peptide Array Kinome Data.
Y. Li, R. J. Arsenault, B. Trost, J. Slind, P. J. Griebel, S. Napper, and A. Kusalik (2012)
Science Signaling 5, pl2
   Abstract »    Full Text »    PDF »
EphB Receptors Trigger Akt Activation and Suppress Fas Receptor-Induced Apoptosis in Malignant T Lymphocytes.
A. Maddigan, L. Truitt, R. Arsenault, T. Freywald, O. Allonby, J. Dean, A. Narendran, J. Xiang, A. Weng, S. Napper, et al. (2011)
J. Immunol. 187, 5983-5994
   Abstract »    Full Text »    PDF »
Computational prediction of eukaryotic phosphorylation sites.
B. Trost and A. Kusalik (2011)
Bioinformatics 27, 2927-2935
   Abstract »    Full Text »    PDF »
Inflammatory Cytokines IL-32 and IL-17 Have Common Signaling Intermediates despite Differential Dependence on TNF-Receptor 1.
E. Turner-Brannen, K.-Y. G. Choi, R. Arsenault, H. El-Gabalawy, S. Napper, and N. Mookherjee (2011)
J. Immunol. 186, 7127-7135
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882