Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 14 April 2009
Vol. 2, Issue 66, p. ra16
[DOI: 10.1126/scisignal.2000098]

RESEARCH ARTICLES

Mechanical Signals Trigger Myosin II Redistribution and Mesoderm Invagination in Drosophila Embryos

Philippe-Alexandre Pouille*, Padra Ahmadi*, Anne-Christine Brunet, and Emmanuel Farge{dagger}

UMR 168, CNRS, Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.

* These authors contributed equally to this work.

Abstract: During Drosophila gastrulation, two waves of constriction occur in the apical ventral cells, leading to mesoderm invagination. The first constriction wave is a stochastic process mediated by the constriction of 40% of randomly positioned mesodermal cells and is controlled by the transcription factor Snail. The second constriction wave immediately follows and involves the other 60% of the mesodermal cells. The second wave is controlled by the transcription factor Twist and requires the secreted protein Fog. Complete mesoderm invagination requires redistribution of the motor protein Myosin II to the apical side of the constricting cells. We show that apical redistribution of Myosin II and mesoderm invagination, both of which are impaired in snail homozygous mutants that are defective in both constriction waves, are rescued by local mechanical deformation of the mesoderm with a micromanipulated needle. Mechanical deformation appears to promote Fog-dependent signaling by inhibiting Fog endocytosis. We propose that the mechanical tissue deformation that occurs during the Snail-dependent stochastic phase is necessary for the Fog-dependent signaling that mediates the second collective constriction wave.

{dagger} To whom correspondence should be addressed. E-mail: efarge{at}curie.fr

Citation: P.-A. Pouille, P. Ahmadi, A.-C. Brunet, E. Farge, Mechanical Signals Trigger Myosin II Redistribution and Mesoderm Invagination in Drosophila Embryos. Sci. Signal. 2, ra16 (2009).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Recapitulation of morphogenetic cell shape changes enables wound re-epithelialisation.
W. Razzell, W. Wood, and P. Martin (2014)
Development 141, 1814-1820
   Abstract »    Full Text »    PDF »
Subcellular and supracellular mechanical stress prescribes cytoskeleton behavior in Arabidopsis cotyledon pavement cells.
A. Sampathkumar, P. Krupinski, R. Wightman, P. Milani, A. Berquand, A. Boudaoud, O. Hamant, H. Jonsson, and E. M. Meyerowitz (2014)
eLife Sci 3, e01967
   Abstract »    Full Text »    PDF »
Patched1 is required in neural crest cells for the prevention of orofacial clefts.
V. Metzis, A. D. Courtney, M. C. Kerr, C. Ferguson, M. C. Rondon Galeano, R. G. Parton, B. J. Wainwright, and C. Wicking (2013)
Hum. Mol. Genet. 22, 5026-5035
   Abstract »    Full Text »    PDF »
The mechanical anisotropy in a tissue promotes ordering in hexagonal cell packing.
K. Sugimura and S. Ishihara (2013)
Development 140, 4091-4101
   Abstract »    Full Text »    PDF »
Signalling through mechanical inputs - a coordinated process.
H. Zhang and M. Labouesse (2012)
J. Cell Sci. 125, 3039-3049
   Abstract »    Full Text »    PDF »
Mechanosensitive mechanisms in transcriptional regulation.
A. Mammoto, T. Mammoto, and D. E. Ingber (2012)
J. Cell Sci. 125, 3061-3073
   Abstract »    Full Text »    PDF »
Not just inductive: a crucial mechanical role for the endoderm during heart tube assembly.
V. D. Varner and L. A. Taber (2012)
Development 139, 1680-1690
   Abstract »    Full Text »    PDF »
Embryonic Clutch Control.
W. Razzell and P. Martin (2012)
Science 335, 1181-1182
   Abstract »    Full Text »    PDF »
The extracellular matrix: A dynamic niche in cancer progression.
P. Lu, V. M. Weaver, and Z. Werb (2012)
J. Cell Biol. 196, 395-406
   Abstract »    Full Text »    PDF »
Mechanosensitive shivering of model tissues under controlled aspiration.
K. Guevorkian, D. Gonzalez-Rodriguez, C. Carlier, S. Dufour, and F. Brochard-Wyart (2011)
PNAS 108, 13387-13392
   Abstract »    Full Text »    PDF »
Phosphoinositides in Cell Architecture.
A. Shewan, D. J. Eastburn, and K. Mostov (2011)
Cold Spring Harb Perspect Biol 3, a004796
   Abstract »    Full Text »    PDF »
Integrin adhesion drives the emergent polarization of active cytoskeletal stresses to pattern cell delamination.
C. Meghana, N. Ramdas, F. M. Hameed, M. Rao, G. V. Shivashankar, and M. Narasimha (2011)
PNAS 108, 9107-9112
   Abstract »    Full Text »    PDF »
Fluidization of tissues by cell division and apoptosis.
J. Ranft, M. Basan, J. Elgeti, J.-F. Joanny, J. Prost, and F. Julicher (2010)
PNAS 107, 20863-20868
   Abstract »    Full Text »    PDF »
Mechanical control of tissue and organ development.
T. Mammoto and D. E. Ingber (2010)
Development 137, 1407-1420
   Abstract »    Full Text »    PDF »
Cochlear outer hair cells undergo an apical circumference remodeling constrained by the hair bundle shape.
R. Etournay, L. Lepelletier, J. B. de Monvel, V. Michel, N. Cayet, M. Leibovici, D. Weil, I. Foucher, J.-P. Hardelin, and C. Petit (2010)
Development 137, 1373-1383
   Abstract »    Full Text »    PDF »
Integration of contractile forces during tissue invagination.
A. C. Martin, M. Gelbart, R. Fernandez-Gonzalez, M. Kaschube, and E. F. Wieschaus (2010)
J. Cell Biol. 188, 735-749
   Abstract »    Full Text »    PDF »
Cell Mechanics and Feedback Regulation of Actomyosin Networks.
R. Fernandez-Gonzalez and J. A. Zallen (2009)
Science Signaling 2, pe78
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882