Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 1 February 2000
Vol. 2000, Issue 17, p. tw2
[DOI: 10.1126/stke.2000.17.tw2]


Fertilization Calcium Turns Off MAP Kinase

The MAP kinase pathway is active in unfertilized oocytes where it inhibits progression through the cell cycle prior to fertilization. Fertilization inhibits the MAP kinase cascade and allows entry into the S phase of the cell cycle. Carroll et al. demonstrated that Ca2+ was necessary and sufficient to inhibit MAP kinase and permit DNA replication in sea urchin eggs. MAP kinase was dephosphorylated and its kinase activity was inhibited in a Ca2+-dependent manner, which could be mimicked by the addition of calcium ionophore and blocked by calcium chelators. DNA synthesis could be stimulated by fertilization, treatment with a MAP kinase inhibitor, or treatment with calcium ionophore. Their data suggest that one role for the rise in intracellular calcium following fertilization is to release the brake on mitosis through inhibition of MAP kinase.

Carroll, D.J., Albay, D.T., Hoang, K.M., O'Neill, F.J., Kumano, M., and Foltz, K.R. (2000) The relationship between calcium, mAP kinase, and DNA synthesis in the sea urchin egg at fertilization. Dev. Biol. 217: 179-191. [Abstract]

Citation: Calcium Turns Off MAP Kinase. Sci. STKE 2000, tw2 (2000).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882