Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 22 February 2000
Vol. 2000, Issue 20, p. tw4
[DOI: 10.1126/stke.2000.20.tw4]


Virology Rotavirus Infections Increases Intracellular Calcium

Rotaviruses cause many cases of viral gastroenteritis in children and animals. Brunet et al. found that rotavirus infected CaCo-2 cells exhibited elevated concentration of free intracellular calcium [Ca2+]i, which lead to disorganization of the microvillar actin cytoskeleton. Rotavirus induced disorganization of the microvillar actin cytoskeleton was completely blocked by calcium chelators. The increase in [Ca2+]i occurred via both increased calcium flux across the plasma membrane and increased release from the endoplasmic reticulum. The infected cells exhibited a two-phase alteration in calcium regulation. Part of the increase in [Ca2+]i was due to the actions of a factor secreted by the infected cells, because supernatant from infected cells was capable of increasing [Ca2+]i in uninfected cells by a phospholipase C-dependent mechanism. The authors suggest that the diarrhea associated with Rotavirus infection may arise from the calcium-induced disruption of the microvillae leading to decreased nutrient digestion and absorption.

Brunet, J.-P., Cotte-Laffitte, J., Linxe, C., Quero, A.-M., Géniteau-Legendre, M., and Servin, A. (2000) Rotavirus infection induces an increase in intracellular calcium concentration in human intestinal epithelial cells: Role for microvillar actin alteration. J. Virol. 74: 2323-2332. [Abstract] [Full Text]

Citation: Rotavirus Infections Increases Intracellular Calcium. Sci. STKE 2000, tw4 (2000).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882