Sci. STKE, 22 February 2000
Ras Ras Dimers in the Membrane
Membrane localization of the small GTP-binding protein Ras is essential for its function. Upon activation, Ras binds to the serine-threonine kinase Raf-1. Inouye et al. suggest that Ras must dimerize at the membrane to activate Raf-1. Purified, GTP-bound Ras that was incorporated into liposomes formed dimers that activated Raf-1 in the absence of other membrane proteins. This effect did not occur in the absence of liposomes. Ras fusion proteins that formed dimers could bypass incorporation into liposomes for Raf-1 activation, and Ras fusion proteins induced to dimerize in intact cells also activated Raf-1. It remains unclear how Ras might dimerize in the membrane.
Citation: Ras Dimers in the Membrane. Sci. STKE 2000, tw6 (2000).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882