Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 14 March 2000
Vol. 2000, Issue 23, p. tw2
[DOI: 10.1126/stke.2000.23.tw2]


Cell Differentiation Interleukin-12 Receptor β2 Chain and TH2 Cells

Differentiation of naïve CD4+ T cells into the T helper type 1 (TH1) phenotype requires signaling by interleukin (IL)-12 through the IL-12 receptor β2 chain, and activation of the signal transducer and activator of transcription (STAT) 4. On the other hand, induction of the TH2 phenotype from naïve CD4+ T cells requires IL-4 binding of the functional IL-4 receptor and activation of STAT6. A concomitant reduction of IL-12Rβ2 is also observed. When naïve CD4+ T cells are treated simultaneously with both IL-4 and IL-12, the IL-4 effects predominate and TH2 cells develop. Thus, it has been hypothesized that IL-4-dependent decreases in IL-12Rβ2 expression prevent IL-12 signals, and this allows for differentiation into the TH2 phenotype. However, it is not known what effect constitutive expression of the IL-12Rβ2 chain has on the development of TH2 cells. Nishikomori et al. found that IL-12Rβ2 transgenic CD4+ T cells differentiated into TH2 cells in the presence of IL-4, or IL-4 and IL-12. IL-12 treatment of TH2 cells expressing functional IL-12 receptors did not induce the conversion of these cells into the TH1 phenotype if the cells were pretreated under TH2 conditions. Additionally, the authors found that the IL-12 receptor-bearing TH2 cells continued to synthesize IL-4 even in the presence of IL-12 in the growth medium. Thus, TH2 cells are resistant to TH1 conversion, and decreased expression of IL-12Rβ2 is not necessary for the establishment of the TH2 phenotype.

Nishikomori, R., Ehrhardt, R.O., and Strober, W. (2000) T helper type 2 cell differentiation occurs in the presence of interleukin-12 receptor β2 chain expression and signaling. J. Exp. Med. 191: 847-858. [Abstract] [Full Text]

Citation: Interleukin-12 Receptor β2 Chain and TH2 Cells. Sci. STKE 2000, tw2 (2000).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882