Sci. STKE, 11 July 2000
Apoptosis Mighty Deadly MyD88
Toll receptors are activated by bacterially produced pathogen-associated molecular pattern (PAMPs). Bacterial lipoproteins (BLPs) are PAMPs that activate the Toll-like receptor 2 (TLR2) through recruitment of the adaptor molecule MyD88. Transfection of a variety of dominant negative mutants allowed Aliprantis et al. to show that MyD88 is a common mediator for both the cell survival signal mediated by NF-B activation and the apoptotic signal mediated through Fas-associated death domain protein (FADD). FADD activation is dependent on the death domain (DD) of MyD88, and the two proteins could be coimmunoprecipitated from transfected cells, confirming a physical interaction between them. Apoptosis was inhibited by mutant forms of caspase 8, FADD, or MyD88, confirming an apoptosis pathway from TLR2 to MyD88 to FADD to caspase 8.
Citation: Mighty Deadly MyD88. Sci. STKE 2000, tw3 (2000).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882