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Sci. STKE, 19 September 2000
Vol. 2000, Issue 50, p. pe1
[DOI: 10.1126/stke.2000.50.pe1]

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Conformational Display: A Role for Switch Polymorphism in the Superfamily of Regulatory GTPases

Stephen R. Sprang

The author is in the Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050, USA. E-mail: sprang@chop.swmed.edu

Abstract: Regulating signal transduction is serious business. If signals are improperly controlled, aberrant proliferation or inappropriate cell death may result. The Ras superfamily of small guanosine triphosphatases (GTPases) and the heterotrimeric G proteins require GTP binding into their active sites for subsequent activation. Hydrolysis of GTP to GDP precedes, and is a requirement for, inactivation. However, the changes in conformation that accompany GTP or GDP binding are now becoming fully appreciated. Sprang discusses the importance of conformation in the activation of GTPases and heterotrimeric G proteins, and the possibility that these proteins exist in several different conformations. Thus, rather than a simplistic on/off two-conformation model, these signaling proteins exist in several differing conformations that may be related to activation and effector protein binding.

Citation: S. R. Sprang, Conformational Display: A Role for Switch Polymorphism in the Superfamily of Regulatory GTPases. Sci. STKE 2000, pe1 (2000).

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