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Sci. STKE, 31 October 2000
Vol. 2000, Issue 56, p. pl1
[DOI: 10.1126/stke.2000.56.pl1]

PROTOCOLS

Application of the Chromatin Immunoprecipitation Method to Identify in Vivo Protein-DNA Associations in Fission Yeast

Kohta Takahashi1*, Shigeaki Saitoh1,2, and Mitsuhiro Yanagida1

1The Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
2Presently at the Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Abstract: The chromatin immunoprecipitation (ChIP) method provides an ideal tool for detecting direct or indirect interactions between proteins of interest and DNAs with known sequences. Here, we introduce the ChIP protocol used in our laboratory to identify in vivo protein-DNA association in the fission yeast Schizosaccharomyces pombe. The cytological and genetic merits of the fission yeast for studying control of the eukaryotic cell cycle and chromosome dynamics are reinforced by application of this ChIP method.

*Corresponding author, E-mail: takahash{at}kozo.biophys.kyoto-u.ac.jp

Citation: K. Takahashi, S. Saitoh, M. Yanagida, Application of the Chromatin Immunoprecipitation Method to Identify in Vivo Protein-DNA Associations in Fission Yeast. Sci. STKE 2000, pl1 (2000).

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