Sci. STKE, 14 November 2000
Cytokine Signaling Dissecting Ceramide Signals
Treatment of cells with the inflammatory cytokine interleukin 1 causes synthesis of ceramide, a sphingolipid metabolite that mediates a signal that causes growth arrest. The growth arrest involves activation of the mitogen-activated protein (MAP) kinase family member SAPK (stress-activated protein kinase). Bourbon et al. analyzed the signaling connection between ceramide and the SAPK pathway in human embryonic kidney cells. Their experiments suggest that ceramide activates protein kinase C (PKC-) and causes association of PKC- with SAPK and also with other members of the SAPK-activating MAP kinase cascade. The authors conclude that PKC- mediates effects of ceramide to cause formation of a multiprotein complex that leads to activation of SAPK.
Bourbon, N.A., Yun, J., and Kester, M. (2000) Ceramide directly activates protein kinase C to regulate a stress-activated protein kinase signaling complex. J. Biol. Chem. 275: 35617-35623. [Full Text] [Abstract]
Citation: Dissecting Ceramide Signals. Sci. STKE 2000, tw6 (2000).
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