Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 19 December 2000
Vol. 2000, Issue 63, p. tw2
[DOI: 10.1126/stke.2000.63.tw2]

EDITORS' CHOICE

Viral Infection Dispensable PKR

Double-stranded RNA (dsRNA) is detected by cells and triggers several different cellular responses. These pathways are important for controlling viral infections in the host and include stimulation of apoptosis to prevent spread of the virus, inhibition of protein synthesis to prevent viral replication, and induction of antiviral interferons and cytokines to stimulate the immune system (which requires suppression of apoptosis). Although protein kinase R (PKR) has been implicated in each of the dsRNA-triggered processes, Iordanov et al. found that the stimulation of the anti-apoptotic nuclear factor {kappa}B (NF-{kappa}B) pathway and the production of β-interferon, tumor necrosis factor-α, and interleukin-6 did not depend on PKR. Comparison of the phosphorylation and degradation of I{kappa}Bs (inhibitors of NF-{kappa}B), nuclear translocation of NF-{kappa}B, and DNA-binding by NF-{kappa}B in wild-type and PKR-deficient mouse embryo fibroblasts in response to dsRNA showed that NF-{kappa}B activation proceeded normally in wild-type and PKR-deficient cells. Stimulation of cytokines and interferons occurred in both the wild-type and PKR mutant cells. The authors propose that the anti-apoptotic responses triggered by dsRNA are controlled by a second sensor that is independent of PKR.

Iordanov, M.S., Wong, J., Bell, J.C., and Magun, B.E. (2000) Activation of NF-{kappa}B by double-stranded RNA (dsRNA) in the absence of protein kinase R and RNase L demonstrates the existence of two separate dsRNA-triggered antiviral programs. Mol. Cell. Biol. 21: 61-72. [Abstract] [Full Text]

Citation: Dispensable PKR. Sci. STKE 2000, tw2 (2000).


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882