Sci. STKE, 2 October 2001
Chromatin Accessibility A Tale of Histone's Tails
The DNA of all eukaryotes is packaged into chromatin, which is composed largely of histone proteins. Covalent modifications of the tails of these proteins play an important role both in chromosome organization and in the specific regulation of individual genes. Litt et al. and Noma et al. show that methylation of Lys4 in the amino-terminal tail of histone H3 is specific to euchromatic domains (where genes are generally active) and methylation of H3 Lys9 is specific to heterochromatic domains of chromatin (where genes are generally inactive). Inverted repeats flanking the heterochromatin act as boundary elements and prevent its spread into the surrounding euchromatic regions. At the level of individual genes, Lo et al. show that phosphorylation of Ser10 on histone H3 by Snf1 kinase facilitates acetylation of Lys14 by Gcn5 (but not vice versa), and that both are needed for the activation of the INO1 gene in vivo.
M. D. Litt, M. Simpson, M. Gaszner, C. D. Allis, G. Felsenfeld, Correlation between histone lysine methylation and developmental changes at the chicken β-globin locus. Science 293, 2453-2455 (2001). [Abstract] [Full Text]
W.-S. Lo, L. Duggan, N. C. Tolga Emre, R. Belotserkovskya, W. S. Lane, R. Shiekhattar, S. L. Berger, Snf1--a histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription. Science 293, 1142-1146 (2001). [Abstract] [Full Text]
Citation: A Tale of Histone's Tails. Sci. STKE 2001, tw362 (2001).