Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 16 October 2001
Vol. 2001, Issue 104, p. tw381
[DOI: 10.1126/stke.2001.104.tw381]


Transcription HOX Proteins as Pseudosubstrates for HATs

The HOX homeodomain is a large family of transcription factors involved in gene regulation, both as transcriptional activators and repressors, at many stages in development. Interactions with other proteins, such as PBC homeodomain proteins, can increase HOX protein affinity and specificity for DNA binding. Shen et al. show that HOX proteins also interact with CBP, a transcriptional regulator with histone acetyltransferase activity. Different members of the HOX family interacted with CBP through the NH2-terminus and the homeodomain, which has a putative consensus acetylation motif. However, the HOX proteins were not acetylated by CBP, instead they inhibited CBP HAT activity in vitro and in vivo. In vivo, this inhibition blocked CBP-mediated increases in reporter gene expression. Addition of CBP to HOX, PBC, and DNA complexes promoted the disassociation of the HOX from DNA based on electrophoretic mobility gel shift assays. Shen et al. suggest that the repressive effects of HOX proteins may be the result of inhibition of CBP HAT activity.

W.-f. Shen, K. Krishnan, H. J. Lawrence, C. Largman, The HOX homeodomain proteins block CBP histone acetyltransferase activity. Mol. Cell. Biol. 21, 7509-7522 (2001). [Abstract] [Full Text]

Citation: HOX Proteins as Pseudosubstrates for HATs. Sci. STKE 2001, tw381 (2001).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882