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Sci. STKE, 27 March 2001
Vol. 2001, Issue 75, p. tw7
[DOI: 10.1126/stke.2001.75.tw7]


Cell Biology Oocyte Maturation and G Beta and Gamma Subunits

The molecular details of progesterone-dependent Xenopus oocyte maturation are not well defined, but adenylyl cyclase activity is inhibited and cyclic AMP (cAMP) concentrations decrease. Maturation does not appear to involve Gαi activity; however, Sheng et al. found that Gβ{gamma} subunits are involved in arresting oocyte meiosis. maturation was induced by overexpressing in oocytes Gβ{gamma} scavengers, such as the α subunit of bovine transducin (Gαt) or a membrane-localized COOH-terminal bovine β-adrenergic receptor kinase (βARK-CCAAX) fragment, both of which sequester Gβ{gamma} subunits. βARK-CCAAX coprecipitated with Gβ in oocyte lysates, suggesting that Gβ-dependent inhibition was perturbed by direct association. Xenopus mitogen-activated protein kinase (xMAPK) was activated, an essential step in maturation, as a consequence of Gβ sequestration. Finally, overexpression of Gβ{gamma} in oocytes expressing βARK-CCAAX inhibited maturation and resulted in increased production of cAMP. These data suggest that Gβ{gamma} may influence oocyte maturation arrest and that progesterone may prevent the activation of a heterotrimeric G protein and the consequent release of Gβ{gamma}.

Y. Sheng, M. Tiberi, R. A. Booth, C. Ma, X. J. Liu, Regulation of Xenopus oocyte meiosis arrest by G protein β{gamma} subunits. Curr. Biol. 11, 405-416 (2001). [Online Journal]

Citation: Oocyte Maturation and G Beta and Gamma Subunits. Sci. STKE 2001, tw7 (2001).

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