Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 15 January 2002
Vol. 2002, Issue 115, p. tw28
[DOI: 10.1126/stke.2002.115.tw28]


Methods Scanning for Phosphotyrosine

Steen et al. report identification of tyrosine phosphorylated peptides and the specific phosphorylated residues using a new method called phosphotyrosine-specific immonium ion (PSI) scanning. Cells treated or not treated with epidermal growth factor (EGF) provided models in which to test the technique. Steen et al. identified tyrosine-phosphorylated proteins and residues already implicated in EGF signaling. In addition, they discovered novel proteins and residues phosphorylated in response to EGF. To their surprise, several of these novel residues were not predicted to be phosphorylated by any of several phosphorylation detection algorithms [Scansite (, ProSite (, and NetPhos (]. Thus, this technique has the power to uncover unexpected and presently unpredictable phosphorylation events.

H. Steen, B. Kuster, M. Fernandez, A. Pandey, M. Mann, Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway. J. Biol. Chem. 277, 1031-1039 (2002). [Abstract] [Full Text]

Citation: Scanning for Phosphotyrosine. Sci. STKE 2002, tw28 (2002).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882