Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 5 February 2002
Vol. 2002, Issue 118, p. tw58
[DOI: 10.1126/stke.2002.118.tw58]

EDITORS' CHOICE

Methods A Green Light for Transgenes

Silencing of retroviral sequences during embryonic development is believed to be a developmental safeguard against uncontrolled expansion of these parasites. However, this shut-off of gene expression has represented a major obstacle for scientists trying to create transgenic animals. Lois et al. have shown that transgenic mice and rats can be generated at high frequencies by infection of single-cell embryos with recombinant lentiviral vectors. The transgene (in this case, green fluorescent protein) was expressed at high levels and could be made tissue-specific by coupling it to an appropriate promoter. The transgene was transmitted through the germ line to the next generation. Although it is not proposed that this approach replace other methods, it may be especially useful for quickly and inexpensively developing many transgenic lines and for other species in which pronuclear injection or other methods have not worked.

C. Lois, E. J. Hong, S. Pease, E. J. Brown, D. Baltimore, Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295, 868-872 (2002). [Abstract] [Full Text]

Citation: A Green Light for Transgenes. Sci. STKE 2002, tw58 (2002).


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882