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Sci. STKE, 16 April 2002
Vol. 2002, Issue 128, p. pl5
[DOI: 10.1126/stke.2002.128.pl5]

PROTOCOLS

Flow Cytometric Analysis of T Cell Receptor Signal Transduction

Traci Zell* and Marc K. Jenkins

University of Minnesota, Department of Microbiology, Room 6-220, Basic Sciences and Biomedical Engineering, 312 Church Street, SE, Minneapolis, MN 55455, USA.

Abstract: Intracellular assays of signal transduction in T cells stimulated by antigen in vivo have been limited by requirements for large numbers of highly purified cells to perform conventional biochemical techniques. Here, we describe a technique using flow cytometry to study signaling events in T cells after antigen recognition in the body.

*Corresponding author. Telephone, 612-626-1188; fax, 612-625-2199; e-mail, zellx001{at}gold.tc.umn.edu

Citation: T. Zell, M. K. Jenkins, Flow Cytometric Analysis of T Cell Receptor Signal Transduction. Sci. STKE 2002, pl5 (2002).

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Science Signaling. ISSN 1937-9145 (pre-2008: Science's STKE. ISSN 1525-8882)