Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 23 April 2002
Vol. 2002, Issue 129, p. tw150
[DOI: 10.1126/stke.2002.129.tw150]


Methods Persistently Interfering

RNA interference (RNAi), in which short, double-stranded RNAs of 21 to 25 nucleotides result in the destruction of messenger (and other) RNAs bearing homologous sequences, has become a powerful tool in the experimental manipulation of gene expression. Results have already demonstrated the efficacy of transiently knocking down or knocking out gene expression in mammalian cells using RNAi. Brummelkamp et al. have now developed a vector system that can direct the persistent synthesis of small, interfering RNAs in mammalian cells and can efficiently suppress gene expression for months.

T. R. Brummelkamp, R. Bernards, R. Agami, A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553 (2002). [Abstract] [Full Text]

Citation: Persistently Interfering. Sci. STKE 2002, tw150 (2002).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882