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Sci. STKE, 7 May 2002
Vol. 2002, Issue 131, p. pe23
Sticky Caveats in an Otherwise Glowing Report: Oligomerizing Fluorescent Proteins and Their Use in Cell Biology
David A. Zacharias*
Merck Research Laboratories, 3535 General Atomics Court, MRLSDB1 Room 1400, San Diego, CA 92122, USA.
Fluorescent proteins from sea creatures have revolutionized the study of cell biology and signal transduction in many ways. Zacharias discusses some of the technical caveats to working with these proteins when they are fused to cellular proteins to track protein localization and interactions. Special attention is paid to problems arising from oligomerization of these fluorescent proteins and how that impacts protein interactions detected by fluorescence resonance energy transfer (FRET).
Tamas Balla and Péter Várnai (26 March 2002) Sci. STKE2002 (125), pl3.
[DOI: 10.1126/scisignal.1252002pl3] |Abstract »|Full Text »|PDF »
Richard M. Siegel, Francis Ka-Ming Chan, David A. Zacharias, Ruth Swofford, Kevin L. Holmes, Roger Y. Tsien, and Michael J. Lenardo (27 June 2000) Sci. STKE2000 (38), pl1.
[DOI: 10.1126/scisignal.382000pl1] |Abstract »|Full Text »|PDF »