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Sci. STKE, 16 July 2002
Vol. 2002, Issue 141, p. tw256
[DOI: 10.1126/stke.2002.141.tw256]


Integrins PKC{epsilon} Controls Intracellular Movement

Integrins mediate cell adhesion and signaling events. The turnover and movement of adhesion sites is critical for cell migration. Ivaska et al. examined how protein kinase C {epsilon} (PKC{epsilon}) influenced cell migration and B1 integrins in mouse embryonic fibroblasts deficient in PKC{epsilon} (PKC{epsilon}KO). Cell lines in which PCK{epsilon} had been reintroduced (PKC{epsilon}RE) exhibited more migratory behavior and more peripheral focal adhesions than did PKC{epsilon}KO cells. The increased migration toward fibronectin required the kinase activity of PCK{epsilon}. When PKC activity was inhibited in the PKC{epsilon}RE cells by bis-indolylmaleimide I (BIM I), β1 integrin from the plasma membrane, PKC{epsilon}, and a tetraspanin protein CD81 accumulated in intracellular vesicles, suggesting that PKC{epsilon} regulates a step in integrin endocytosis and intracellular trafficking. Cells from the PKC{epsilon}KO did not show this same redistribution of integrins; however, after transfection of the kinase-inactive mutant of PKC{epsilon} vesicular localization was detected. Thus, absence of PCK{epsilon} may not be equivalent to inhibition of PKC{epsilon}. In vitro, release of PKC{epsilon} from vesicles isolated after BIM I treatment required energy and PKC{epsilon} kinase activity, suggesting that PKC{epsilon} activity regulates recycling from this compartment.

J. Ivaska, R. D. H. Whelan, R. Watson, P. J. Parker, PKC{epsilon} controls the traffic of β1 integrins in motile cells. EMBO J. 21, 3608-3619 (2002). [Abstract] [Full Text]

Citation: PKC{epsilon} Controls Intracellular Movement. Sci. STKE 2002, tw256 (2002).

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