|
Sci. STKE, 10 September 2002 PROTOCOLSEnumeration, Phenotyping, and Identification of Activation Events in Conjugates Between T cells and Antigen-Presenting Cells by Flow CytometryKristie M. Grebe and Terry A. Potter* Integrated Department of Immunology, National Jewish Medical and Research Center and the University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA. Abstract: Microscopic analysis of T cell-antigen-presenting cell (T cell:APC) interactions at the single cell level has been a powerful, but tedious and subjective, technique. In this paper, we describe a rapid and quantitative method to identify T cell:APC conjugates using succinimidyl ester dyes, which irreversibly label free amine groups on the cell surface. The labeled cell conjugates and subsequent activation events are detected by flow cytometry. *Corresponding author. E-mail, pottert{at}njc.org
Citation: K. M. Grebe, T. A. Potter, Enumeration, Phenotyping, and Identification of Activation Events in Conjugates Between T cells and Antigen-Presenting Cells by Flow Cytometry. Sci. STKE 2002, pl14 (2002). THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
|
Science Signaling. ISSN 1937-9145 (pre-2008: Science's STKE. ISSN 1525-8882)
Magazine | News | Signaling | Careers | Multimedia | Collections | Help | Site Map | RSS
Subscribe | Feedback | Privacy / Legal | About Us | Advertise With Us | Contact Us
© 2002 American Association for the Advancement of Science. All Rights Reserved.
AAAS is a partner of HINARI, AGORA, PatientInform, CrossRef, and COUNTER.
You have reached the bottom of the page. Back to top