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Sci. STKE, 10 September 2002
Vol. 2002, Issue 149, p. pl14
[DOI: 10.1126/stke.2002.149.pl14]

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Enumeration, Phenotyping, and Identification of Activation Events in Conjugates Between T cells and Antigen-Presenting Cells by Flow Cytometry

Kristie M. Grebe and Terry A. Potter*

Integrated Department of Immunology, National Jewish Medical and Research Center and the University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA.

Abstract: Microscopic analysis of T cell-antigen-presenting cell (T cell:APC) interactions at the single cell level has been a powerful, but tedious and subjective, technique. In this paper, we describe a rapid and quantitative method to identify T cell:APC conjugates using succinimidyl ester dyes, which irreversibly label free amine groups on the cell surface. The labeled cell conjugates and subsequent activation events are detected by flow cytometry.

*Corresponding author. E-mail, pottert{at}njc.org

Citation: K. M. Grebe, T. A. Potter, Enumeration, Phenotyping, and Identification of Activation Events in Conjugates Between T cells and Antigen-Presenting Cells by Flow Cytometry. Sci. STKE 2002, pl14 (2002).

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