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Sci. STKE, 10 December 2002
Vol. 2002, Issue 162, p. pl18
[DOI: 10.1126/stke.2002.162.pl18]

PROTOCOLS

Design and Use of a Mammalian Protein-Protein Interaction Trap (MAPPIT)

Sven Eyckerman, Irma Lemmens, Sam Lievens, Jose Van der Heyden, Annick Verhee, Joël Vandekerckhove, and Jan Tavernier*

Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Research (VIB09), Ghent University, Faculty of Medicine and Health Sciences, A. Baertsoenkaai 3, B-9000 Ghent, Belgium.

Abstract: Identifying the interaction partners of a protein is a straightforward way to gain insight into the protein's function and to position it in an interaction network such as a signal transduction pathway. Various techniques have been developed to serve this purpose, and some are specifically designed to study posttranslational modifications in mammalian proteins and to clarify their normal physiological context. However, several intrinsic constraints limit the use of these technologies, and most are not suitable for screening for new interacting partners. In the Mammalian Protein-Protein Interaction Trap (MAPPIT) Protocol described here, knowledge of cytokine receptor signaling has been used to design a versatile genetic tool that can be used analytically and for detection of new protein-protein interactions in mammalian cells.

*Corresponding author. E-mail, jan.tavernier{at}rug.ac.be

Citation: S. Eyckerman, I. Lemmens, S. Lievens, J. Van der Heyden, A. Verhee, J. Vandekerckhove, J. Tavernier, Design and Use of a Mammalian Protein-Protein Interaction Trap (MAPPIT). Sci. STKE 2002, pl18 (2002).

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