Sci. STKE, 10 June 2003
CHECKPOINT SIGNALS Seeking out Single-Stranded DNA
The checkpoint signals that cells generate in response to DNA damage are mediated by the ATR protein kinase, which functions in a complex with the ATRIP protein. Zou and Elledge (see the Perspective by Carr) found that the ATR-ATRIP complex was not recruited to sites of DNA damage in cells that lacked replication protein A (RPA). Although RPA functions in processes such as replication and recombination, it also appears to initiate the checkpoint signal by binding to single-stranded DNA and then interacting with the ATR-ATRIP complex, which phosphorylates substrate proteins at the damage site.
Citation: Seeking out Single-Stranded DNA. Sci. STKE 2003, tw222 (2003).
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