Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Sci. STKE, 15 July 2003
Vol. 2003, Issue 191, p. tr1
[DOI: 10.1126/stke.2003.191.tr1]

TEACHING RESOURCES

Activation of Ras by Grb2-SOS: Demonstrating an Assembly Role of SH3 Domains

Ali Zarrinpar1,2, Roby P. Bhattacharyya1,2, Wendell A. Lim2, and Nancy R. Gough3*

1Program in Biological Sciences, University of California San Francisco, 600 16th Street, San Francisco, CA 94143-2240, USA.
2Department of Cellular and Molecular Pharmacology and Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94143-2240, USA.
3Science's STKE, American Association for the Advancement of Science, 1200 New York Avenue, N.W., Washington, DC 20005, USA.

Abstract: The animation shows a schematic representation of the assembly role of SH3 domain-containing proteins using Grb2 as an example. Growth factor stimulation leads to the activation of receptor tyrosine kinases and to the phosphorylation of the receptor tail and the stimulation of phosphorylation of related adaptor proteins (not shown). The resultant phosphotyrosines form docking sites for the adaptor protein Grb2 (through its SH2 domain). The Grb2 SH3 domains bind proline-rich motifs in SOS, the guanine nucleotide exchange factor for Ras, recruiting SOS to the membrane and colocalizing it with Ras. The resultant stimulation of Ras activates a mitogen-activated protein kinase cascade, leading to cell growth and differentiation. The animation could be useful in demonstrating how different protein domains allow dynamic regulation of protein activity, protein assembly, and protein recruitment, allowing cells to respond to signals from their extracellular environment.

[Resource Details]

*Corresponding author. E-mail: ngough{at}aaas.org

Citation: A. Zarrinpar, R. P. Bhattacharyya, W. A. Lim, N. R. Gough, Activation of Ras by Grb2-SOS: Demonstrating an Assembly Role of SH3 Domains. Sci. STKE 2003, tr1 (2003).

ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (pre-2008: Science's STKE. ISSN 1525-8882)