Sci. STKE, 5 August 2003
PLANT BIOLOGY Understanding Ethylene
The development of tools that help in the functional characterization of genes is important as genomic advances continue. Alonso et al. developed a genome-wide collection of sequence-indexed insertion mutants in the model plant Arabidopsis thaliana. Nearly 2/3 of the total complement of Arabidopsis genes were individually knocked out by generating over 225,000 insertional mutations with Agrobacterium transferred DNA. Integration did not occur randomly at the chromosome or gene level. The authors have used this resource to identify new genes that are involved in responses to the plant hormone ethylene.
J. M. Alonso, A. N. Stepanova, T. J. Leisse, C. J. Kim, H. Chen, P. Shinn, D. K. Stevenson, J. Zimmerman, P. Barajas, R. Cheuk, C. Gadrinab, C. Heller, A. Jeske, E. Koesema, C. C. Meyers, H. Parker, L. Prednis, Y. Ansari, N. Choy, H. Deen, M. Geralt, N. Hazari, E. Hom, M. Karnes, C. Mulholland, R. Ndubaku, I. Schmidt, P. Guzman, L. Aguilar-Henonin, M. Schmid, D. Weigel, D. E. Carter, T. Marchand, E. Risseeuw, D. Brogden, A. Zeko, W. L. Crosby, C. C. Berry, J. R. Ecker, Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301, 653-657 (2003). [Abstract] [Full Text]
Citation: Understanding Ethylene. Sci. STKE 2003, tw308 (2003).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882