Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 2 September 2003
Vol. 2003, Issue 198, p. tw337
[DOI: 10.1126/stke.2003.198.tw337]

EDITORS' CHOICE

DEVELOPMENTAL BIOLOGY Behavior and Vulval Development

Development of the vulva of the nematode Caenorhabditis elegans is a model for studying cell fate signaling. The epidermal growth factor receptor-like tyrosine kinase (LET-23), which stimulates a Ras-mitogen-activated protein kinase pathway, as well as the Notch-like pathway and the Wnt pathway, contributes to vulval development. A gain-of-function mutant of the Gαq homolog, EGL-30, exhibited rare ectopic vulval tissue. These worms also exhibit hyperactive locomotor and egg-laying behaviors. Moghal et al. used genetic interactions and targeted gene expression to show that EGL-30 expressed in motor neurons promoted vulval induction. The ability of EGL-30 to promote vulval development required the L-type voltage-gated calcium channel (EGL-19) expressed in body wall muscles. EGL-30 did not appear to be acting through the LET-23 pathway; however, EGL-30 vulval development was inhibited by loss of β-catenin (BAR-1) of the Wnt pathway. Consistent with behavior modulating vulval development, loss-of-function mutations in egl-30 did not affect vulval development of worms grown on solid media, but did inhibit vulval development of worms grown in liquid culture. In liquid, the worms exhibit a dramatically more vigorous movement than that observed for worms grown on solid media. Thus, the authors propose that behavior changes in response to environmental conditions appear to play a role in cell fate specification.

N. Moghal, L. R. Garcia, L. A. Khan, K. Iwasaki, P. W. Sternberg, Modulation of EGF receptor-mediated vulva development by the heterotrimeric G-protein Gq and excitable cells in C. elegans. Development 130, 4553-4566 (2003). [Abstract] [Full Text]

Citation: Behavior and Vulval Development. Sci. STKE 2003, tw337 (2003).



To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882