Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. STKE, 3 February 2004
Vol. 2004, Issue 218, p. pl1
[DOI: 10.1126/stke.2182004pl1]


Stimulation of Cells Using EGF-Coated Magnetic Beads

Stephan J. Kempiak and Jeffrey E. Segall*

Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA.

Abstract: The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.

*Corresponding author. Telephone, 718-430-4237; fax, 718-430-8996; e-mail, segall{at}

Citation: S. J. Kempiak, J. E. Segall, Stimulation of Cells Using EGF-Coated Magnetic Beads. Sci. STKE 2004, pl1 (2004).

Read the Full Text

WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells.
C. Sarmiento, W. Wang, A. Dovas, H. Yamaguchi, M. Sidani, M. El-Sibai, V. DesMarais, H. A. Holman, S. Kitchen, J. M. Backer, et al. (2008)
J. Cell Biol. 180, 1245-1260
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882