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Sci. STKE, 3 February 2004
Vol. 2004, Issue 218, p. pl1
[DOI: 10.1126/stke.2182004pl1]

PROTOCOLS

Stimulation of Cells Using EGF-Coated Magnetic Beads

Stephan J. Kempiak and Jeffrey E. Segall*

Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA.

Abstract: The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.

*Corresponding author. Telephone, 718-430-4237; fax, 718-430-8996; e-mail, segall{at}aecom.yu.edu

Citation: S. J. Kempiak, J. E. Segall, Stimulation of Cells Using EGF-Coated Magnetic Beads. Sci. STKE 2004, pl1 (2004).

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