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Sci. STKE, 10 February 2004
Vol. 2004, Issue 219, p. pl5
Imaging Calcium Concentration Dynamics in Small Neuronal Compartments
Esther A. Nimchinsky1,3,
Thomas A. Pologruto1,2,
Thomas G. Oertner1,4,
Bernardo L. Sabatini1,5, and
1Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA. 2Graduate Program in Biophysics, Harvard University, Cambridge, MA 02138, USA. 3Present address: Center for Molecular and Behavioral Neuroscience Aidekman Research Center, Rutgers State University, Newark Campus, 197 University Avenue, Newark, NJ 07102, USA. 4Present address: Friedrich Miescher Institute, WRO-1066.4.10, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. 5Present address: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.
Citation: R. Yasuda, E. A. Nimchinsky, V. Scheuss, T. A. Pologruto, T. G. Oertner, B. L. Sabatini, K. Svoboda, Imaging Calcium Concentration Dynamics in Small Neuronal Compartments. Sci. STKE2004, pl5 (2004).
Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design.
J. Akerboom, J. D. V. Rivera, M. M. R. Guilbe, E. C. A. Malave, H. H. Hernandez, L. Tian, S. A. Hires, J. S. Marvin, L. L. Looger, and E. R. Schreiter (2009)
J. Biol. Chem.
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