Sci. STKE, 22 June 2004
MICROBIOLOGY Forming Safe Havens Within
The propagation of invasive bacteria and mycobacteria is tightly linked to their ability to withstand delivery to the degradative compartment of the cell, the lysosome. Walburger et al. identify a mycobacterial protein involved in the modulation of phagosome-lysosome trafficking. Protein kinase G, a eukaryotic-like serine/threonine kinase from the pathogenic mycobacterium Mycobacterium tuberculosis, is responsible for inhibiting phagosome-lysosome fusion, promoting mycobacterial survival inside macrophages. Hernandez et al. find that Salmonella, the food-poisoning and typhoid fever bacterium, modulates vesicular trafficking by altering phosphoinositide metabolism through SopB, a phosphoinositide phosphatase. SopB is delivered into host cells by an invasion-associated specialized secretion system and mediates the formation of a characteristic very spacious phagosome within which Salmonella resides after internalization. In the absence of SopB, invading Salmonella reside within tight phagosomes and exhibit a membrane trafficking defect and impaired bacterial intracellular growth.
A. Walburger, A. Koul, G. Ferrari, L. Nguyen, C. Prescianotto-Baschong, K. Huygen, B. Klebl, C. Thompson, G. Bacher, J. Pieters, Protein kinase G from pathogenic mycobacteria promotes survival within macrophages. Science 304, 1800-1804 (2004). [Abstract] [Full Text]
Citation: Forming Safe Havens Within. Sci. STKE 2004, tw226 (2004).
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