Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 5 October 2004
Vol. 2004, Issue 253, p. tw354
[DOI: 10.1126/stke.2532004tw354]


CELL ADHESION Cleaving the Lynch Pin

Franco et al. examined the role of talin proteolysis by calpain, a calcium-dependent protease, in controlling adhesion complex stability. The dynamic formation and disassembly of these complexes is essential for cell motility. In cultured cells exposed to siRNA to decrease calpain 2 abundance, adhesion complexes were more stable and talin cleavage was inhibited. The authors analyzed adhesion complex stability in talin-deficient cells expressing a green fluorescent protein (GFP)-tagged form of talin either with or without a mutation that prevented calpain cleavage. In cells expressing the cleavage-deficient form of talin, adhesion complexes were more stable, exhibiting a decreased rate of disassembly. In addition, the adhesion complexes that contained the noncleavable form of talin were larger and elongated compared with those complexes containing wild-type talin. The authors speculate that because the talin cleavage products still bound some of their complex partners, for example, focal adhesion-targeted type I phosphatidylinositol phosphate kinase (PIPKI{gamma}661), redistribution of the talin cleavage products may also promote redistribution of other adhesion complex proteins, thereby facilitating adhesion complex disassembly.

S. J. Franco, M. A. Rodgers, B. J. Perrin, J. Han, D. A. Bennin, D. R. Critchley, A. Huttenlocher, Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 6, 977-983 (2004). [Online Journal]

Citation: Cleaving the Lynch Pin. Sci. STKE 2004, tw354 (2004).

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882